Detection of rotavirus-specific IgG antibodies by immunoperoxidase assay and enzyme-linked immunosorbent assay

Bat Sheva Zentner, Miriam Margalith, Aaron Galil, Benjamin Halevy, Israel Sarov

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

An indirect immunoperoxidase assay (IPA) has been developed for determination of IgG antibodies to rotavirus. The technique employed as antigen, SA-11 infected MA 104 cells, which were air-dried on glass slides and acetone-fixed. In parallel, rota-specific IgG antibodies were determined by enzyme-linked immunosorbent assay (ELISA). Specific IgG antibodies to rotavirus were determined in sera of healthy children and in sera of patients suffering from gastroenteritis. A good correlation (r = 0.92)and(r = 0.98) for healthy children and patients, respectively, was found between IPA and ELISA techniques. The IPA technique is rapid and simple and positive results, because of the intensive staining, are easily read by low-power light microscope. The potential application of IPA and ELISA methods in serodiagnosis of rotavirus infections is discussed.

Original languageEnglish
Pages (from-to)199-206
Number of pages8
JournalJournal of Virological Methods
Volume11
Issue number3
DOIs
StatePublished - 1 Jan 1985

Keywords

  • IgG
  • enzyme-linked immunosorbent assay
  • immunoperoxidase
  • rotavirus

ASJC Scopus subject areas

  • Virology

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