Detection of tumor mutant APC DNA in plasma of patients with sporadic colorectal cancer

E. Ling, A. Fich, S. Man, M. Wolfson, R. Mikhailowsky, S. A. Lamprecht

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

The aim of this study was to detect mutant APC DNA of tumor origin in the plasma of patients with sporadic colorectal carcinomas. The polymerase chain reaction (PCR) and the single strand conformation polymorphism (SSCP) procedures were employed to detect DNA alterations using primers to amplify the mutation cluster region of the APC gene. APC mutations were observed in 7 out of 11 archival colonic tumor specimens examined. Matching mutations in free plasma DNA of tumor origin were detected in 3 of the 7 patients (42.8%). The results of this preliminary report indicated the presence of APC DNA in plasma harboring the identical abnormal molecular signature of tumor APC DNA. Detection methods of mutant APC DNA in blood may prove useful in the screening and monitoring of patients at risk of or with colorectal cancer.

Original languageEnglish
Pages (from-to)543-546
Number of pages4
JournalIn Vivo
Volume14
Issue number4
StatePublished - 1 Jan 2000

Keywords

  • APC mutations
  • Colon cancer
  • DNA
  • PCR
  • Plasma
  • SSCP

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology
  • Pharmacology

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