Determination of fatty acid profiles and TAGs in vegetable oils by MALDI-TOF/MS fingerprinting

Zeev Wiesman, Bishnu P. Chapagain

Research output: Contribution to journalArticlepeer-review

17 Scopus citations


Vegetable oils are complex mixtures containing a wide range of major compounds. Triacylglycerols (TAGs; consisting of a glycerol moiety with each hydroxyl group esterified to a fatty acid) are the major components (95–98%) of vegetable oils. TAGs are an important source of energy and nutrition for humans, so their compositional analysis merits extensive interest. Analysis of TAGs has increased in recent years and the advancement has been driven by the development of analytical technologies. This chapter discusses techniques for determination of TAG and fatty acid profiles (FAPs) of vegetable oils using matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF/ MS) and gas chromatography–mass spectrometry (GC/MS). Considering the importance of TAG in its native form, rather than FAPs, special emphasis has been given to the TAG fingerprinting analyses of intact oils. MALDI-TOF/MS also enabled calculation of the main fatty acids and their compositions in a simple manner from the TAG profiles; the results are found to be very similar to the prevailing methods of derivatization using GC/MS. This study depicts the potential of MALDI-TOF/MS as an easy, fast, and reliable technique to characterize the TAG and FAPs in vegetable oils.

Original languageEnglish
Pages (from-to)315-336
Number of pages22
JournalMethods in Molecular Biology
StatePublished - 1 Jan 2009


  • Fatty acid profiles
  • GC/MS
  • Triacylglycerols
  • Vegetable oils

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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