Development and testing of a smartphone adapter for image quality assurance and whole slide imaging

Aliyah Arunasalam; Kfir Bar-Am; Cathy Sebag; Whitney Elminger; Amir Bernat; Frank Bolton; David Levitz

doi:10.1117/12.2511137

Development and testing of a smartphone adapter for image quality assurance and whole slide imaging

Aliyah Arunasalam, Kfir Bar-Am, Cathy Sebag, Whitney Elminger, Amir Bernat, Frank Bolton, David Levitz

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

Background: When running large trials, histopathology services are used to assess the state of a tissue. However, in many clinics in low resource settings there are large variations in quality of such services, specifically in biopsy processing and histopathological interpretation/assessment of images. Quality assurance (QA) is needed, but it involves physically mailing slides to a remote clinic. A telemedicine solution can address this challenge. Methods: A novel smartphone adapter for microscopes was developed, consisting of a 3D printed attachment and software integration for the image capture. The attachment is used to couple the eyepiece of a low end microscope to a smartphone (Samsung J530). Image capture was controlled through the EVA System app. The entire system was characterized optically using standard calibration targets. Additionally, images captured on the attachment were compared to the standard method of shipping and scanning slides in a high end slice scanner at a remote clinic. Results: The resolution of the entire system (microscope + phone) with a 40X objective was <1 μm. The system is currently undergoing testing in Nigeria as part of a broader cervical cancer screening study.¹ Preliminary testing showed similar image quality between the smartphone-based system and high end scanner. Whole slide imaging requires stitching together images into a mosaic, made possible by a mobile application. Conclusion: The results here show that coupling a low end microscope to a smartphone yields similar results to a transporting slides to a high end microscope. Such an attachment can thus potentially provide a telemedicine solution to researchers in low resource settings.

Original language	English
Title of host publication	Optics and Biophotonics in Low-Resource Settings V
Editors	David Levitz, Aydogan Ozcan
Publisher	SPIE
ISBN (Electronic)	9781510623804
DOIs	https://doi.org/10.1117/12.2511137
State	Published - 1 Jan 2019
Externally published	Yes
Event	Optics and Biophotonics in Low-Resource Settings V 2019 - San Francisco, United States Duration: 2 Feb 2019 → 3 Feb 2019

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	10869
ISSN (Print)	1605-7422

Conference

Conference	Optics and Biophotonics in Low-Resource Settings V 2019
Country/Territory	United States
City	San Francisco
Period	2/02/19 → 3/02/19

Keywords

Histopathology
Imaging
Low resource settings
Microscopy
Smartphones

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Atomic and Molecular Physics, and Optics
Biomaterials
Radiology Nuclear Medicine and imaging

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1117/12.2511137

Cite this

Arunasalam, A., Bar-Am, K., Sebag, C., Elminger, W., Bernat, A., Bolton, F., & Levitz, D. (2019). Development and testing of a smartphone adapter for image quality assurance and whole slide imaging. In D. Levitz, & A. Ozcan (Eds.), Optics and Biophotonics in Low-Resource Settings V Article 108690O (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 10869). SPIE. https://doi.org/10.1117/12.2511137

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title = "Development and testing of a smartphone adapter for image quality assurance and whole slide imaging",

abstract = "Background: When running large trials, histopathology services are used to assess the state of a tissue. However, in many clinics in low resource settings there are large variations in quality of such services, specifically in biopsy processing and histopathological interpretation/assessment of images. Quality assurance (QA) is needed, but it involves physically mailing slides to a remote clinic. A telemedicine solution can address this challenge. Methods: A novel smartphone adapter for microscopes was developed, consisting of a 3D printed attachment and software integration for the image capture. The attachment is used to couple the eyepiece of a low end microscope to a smartphone (Samsung J530). Image capture was controlled through the EVA System app. The entire system was characterized optically using standard calibration targets. Additionally, images captured on the attachment were compared to the standard method of shipping and scanning slides in a high end slice scanner at a remote clinic. Results: The resolution of the entire system (microscope + phone) with a 40X objective was <1 μm. The system is currently undergoing testing in Nigeria as part of a broader cervical cancer screening study.1 Preliminary testing showed similar image quality between the smartphone-based system and high end scanner. Whole slide imaging requires stitching together images into a mosaic, made possible by a mobile application. Conclusion: The results here show that coupling a low end microscope to a smartphone yields similar results to a transporting slides to a high end microscope. Such an attachment can thus potentially provide a telemedicine solution to researchers in low resource settings.",

keywords = "Histopathology, Imaging, Low resource settings, Microscopy, Smartphones",

author = "Aliyah Arunasalam and Kfir Bar-Am and Cathy Sebag and Whitney Elminger and Amir Bernat and Frank Bolton and David Levitz",

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doi = "10.1117/12.2511137",

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Arunasalam, A, Bar-Am, K, Sebag, C, Elminger, W, Bernat, A, Bolton, F & Levitz, D 2019, Development and testing of a smartphone adapter for image quality assurance and whole slide imaging. in D Levitz & A Ozcan (eds), Optics and Biophotonics in Low-Resource Settings V., 108690O, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 10869, SPIE, Optics and Biophotonics in Low-Resource Settings V 2019, San Francisco, United States, 2/02/19. https://doi.org/10.1117/12.2511137

Development and testing of a smartphone adapter for image quality assurance and whole slide imaging. / Arunasalam, Aliyah; Bar-Am, Kfir; Sebag, Cathy et al.
Optics and Biophotonics in Low-Resource Settings V. ed. / David Levitz; Aydogan Ozcan. SPIE, 2019. 108690O (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 10869).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Development and testing of a smartphone adapter for image quality assurance and whole slide imaging

AU - Arunasalam, Aliyah

AU - Bar-Am, Kfir

AU - Sebag, Cathy

AU - Elminger, Whitney

AU - Bernat, Amir

AU - Bolton, Frank

AU - Levitz, David

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Background: When running large trials, histopathology services are used to assess the state of a tissue. However, in many clinics in low resource settings there are large variations in quality of such services, specifically in biopsy processing and histopathological interpretation/assessment of images. Quality assurance (QA) is needed, but it involves physically mailing slides to a remote clinic. A telemedicine solution can address this challenge. Methods: A novel smartphone adapter for microscopes was developed, consisting of a 3D printed attachment and software integration for the image capture. The attachment is used to couple the eyepiece of a low end microscope to a smartphone (Samsung J530). Image capture was controlled through the EVA System app. The entire system was characterized optically using standard calibration targets. Additionally, images captured on the attachment were compared to the standard method of shipping and scanning slides in a high end slice scanner at a remote clinic. Results: The resolution of the entire system (microscope + phone) with a 40X objective was <1 μm. The system is currently undergoing testing in Nigeria as part of a broader cervical cancer screening study.1 Preliminary testing showed similar image quality between the smartphone-based system and high end scanner. Whole slide imaging requires stitching together images into a mosaic, made possible by a mobile application. Conclusion: The results here show that coupling a low end microscope to a smartphone yields similar results to a transporting slides to a high end microscope. Such an attachment can thus potentially provide a telemedicine solution to researchers in low resource settings.

AB - Background: When running large trials, histopathology services are used to assess the state of a tissue. However, in many clinics in low resource settings there are large variations in quality of such services, specifically in biopsy processing and histopathological interpretation/assessment of images. Quality assurance (QA) is needed, but it involves physically mailing slides to a remote clinic. A telemedicine solution can address this challenge. Methods: A novel smartphone adapter for microscopes was developed, consisting of a 3D printed attachment and software integration for the image capture. The attachment is used to couple the eyepiece of a low end microscope to a smartphone (Samsung J530). Image capture was controlled through the EVA System app. The entire system was characterized optically using standard calibration targets. Additionally, images captured on the attachment were compared to the standard method of shipping and scanning slides in a high end slice scanner at a remote clinic. Results: The resolution of the entire system (microscope + phone) with a 40X objective was <1 μm. The system is currently undergoing testing in Nigeria as part of a broader cervical cancer screening study.1 Preliminary testing showed similar image quality between the smartphone-based system and high end scanner. Whole slide imaging requires stitching together images into a mosaic, made possible by a mobile application. Conclusion: The results here show that coupling a low end microscope to a smartphone yields similar results to a transporting slides to a high end microscope. Such an attachment can thus potentially provide a telemedicine solution to researchers in low resource settings.

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KW - Imaging

KW - Low resource settings

KW - Microscopy

KW - Smartphones

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M3 - Conference contribution

AN - SCOPUS:85064405883

T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

BT - Optics and Biophotonics in Low-Resource Settings V

A2 - Levitz, David

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PB - SPIE

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Y2 - 2 February 2019 through 3 February 2019

ER -

Development and testing of a smartphone adapter for image quality assurance and whole slide imaging

Abstract

Publication series

Conference

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

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