Development of a highly sensitive, field operable biosensor for serological studies of Ebola virus in central Africa

A. Petrosova, T. Konry, S. Cosnier, I. Trakht, J. Lutwama, E. Rwaguma, A. Chepurnov, E. Mühlberger, L. Lobel, R. S. Marks

Research output: Contribution to journalArticlepeer-review

44 Scopus citations

Abstract

We describe herein a newly developed optical immunosensor for detection of antibodies directed against antigens of the Ebola virus strains Zaire and Sudan. We employed a photo immobilization methodology based on a photoactivatable electrogenerated poly(pyrrole-benzophenone) film deposited upon an indium tin oxide (ITO) modified conductive surface fiber-optic. It was then linked to a biological receptor, Ebola virus antigen in this case, on the fiber tip through a light driven reaction. The photochemically modified optical fibers were tested as an immunosensor for detection of antibodies against Ebola virus, in animal and human sera, by use of a coupled chemiluminescent reaction. The immunosensor was tested for sensitivity, specificity, and compared to standard chemiluminescent ELISA under the same conditions. The analyte, anti-Ebola IgG, was detected at a low titer of 1:960,000 and 1:1,000,000 for subtypes Zaire and Sudan, respectively. While the same serum tested by ELISA was one order (24 times) less sensitive.

Original languageEnglish
Pages (from-to)578-586
Number of pages9
JournalSensors and Actuators, B: Chemical
Volume122
Issue number2
DOIs
StatePublished - 26 Mar 2007

Keywords

  • Ebola virus
  • Electropolymerization
  • Immunosensor
  • Optical fibers
  • Poly(pyrrole-benzophenone)

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

Fingerprint

Dive into the research topics of 'Development of a highly sensitive, field operable biosensor for serological studies of Ebola virus in central Africa'. Together they form a unique fingerprint.

Cite this