Development of a porcine hard-to-heal wound model: Evaluation of a bromelain-based enzymatic debriding agent

Yaron Shoham, Itai Sabbag, Adam J. Singer

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Aims: We describe the development of a novel porcine eschar model and compare the debridement efficacy of various concentrations of a novel bromelain-based enzymatic agent with collagenase. Methods: Full thickness excisional wounds were created on pigs and injected intradermally with various doses of doxorubicin. Wounds were monitored for a period of 46 days for the development of eschar and wound closure. After determining the optimal concentration and dose of doxorubicin resulting in non-healing eschars, these conditions were used to create additional wounds on another set of animals. The resulting eschars were treated with various concentrations of a novel bromelain-based enzymatic agent (EscharEx-02) or collagenase. The primary endpoint was greater than 95% removal of the central eschar. Results: Consistent eschars composed of two distinct areas (a central area of exudate and slough representing the hard-toheal wound bed, and a peripheral area of full-thickness mummified necrosis) were seen after injection of doxorubicin (0.5 ml/cm2 of stock solution 0.75mg/ml) at one and six days after wound creation. Complete removal of the central eschar was achieved in all wounds after five and eight treatments with 5% and 2% EscharEx-02 respectively. Complete removal of the central eschar with collagenase was achieved in 0% and 82% of the wounds after 10 and 16 treatments respectively. Conclusions: We describe a porcine model for creating eschars similar to hard-to-heal wounds in humans. A novel bromelain-based enzymatic debridement agent was more effective than a commercially available collagenase in removing eschars in this wound model.

    Original languageEnglish
    Pages (from-to)VIi-VIx
    JournalJournal of wound care
    Volume30
    DOIs
    StatePublished - 2 Sep 2021

    Keywords

    • Bromelain
    • Collagenase
    • Debridement
    • Eschar
    • Hard-to-heal
    • Necrosis
    • Porcine
    • Research
    • Wound
    • Wound healing
    • Wounds

    ASJC Scopus subject areas

    • Fundamentals and skills
    • Nursing (miscellaneous)

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