Development of an "electroptode" immunosensor: Indium tin oxide-coated optical fiber tips conjugated with an electropolymerized thin film with conjugated cholera toxin B subunit

Tania Konry, Andres Novoa, Serge Cosnier, Robert S. Marks

    Research output: Contribution to journalArticlepeer-review

    62 Scopus citations

    Abstract

    We demonstrate that it is possible to create surface-conductive fiber optics, upon which may be electropolymerized a biotinylated polypyrrole thin film, which may then be used to affinity coat the fiber with molecular recognition probes. This fiber-optic electroconductive surface modification is done by the deposition of a thin layer of indium tin oxide. Thereafter, biotin - pyrrole monomers are electropolymerized onto the conductive metal oxide surface and then exposed to avidin. Avidin biotin interactions were used to modify the fiber optics with biotin-conjugated cholera toxin B subunit molecules, for the construction of an immunosensor to detect cholera antitoxin antibodies. The biosensor was tested for sensitivity, nonspecificity, and overall practicality.

    Original languageEnglish
    Pages (from-to)2633-2639
    Number of pages7
    JournalAnalytical Chemistry
    Volume75
    Issue number11
    DOIs
    StatePublished - 1 Jun 2003

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