Developmental expression of the growth hormone gene in the gilthead sea bream Sparus aurata

Bruria Funkenstein, Amos Tandler, Benzion Cavari

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Expression of growth hormone (GH) gene during early stages of larval development of the teleost Sparus aurata was determined by Northern blot analysis. Poly(A+) RNA was prepared from a pool of larvae collected on different days after hatching. When hybridized to Sparus aurata GH cDNA, GH specific mRNA was first seen on day 6 post-hatching. In contrast, the levels of β-actin mRNA, which was used to normalize for RNA amounts, were already high on the day of hatching. Our results suggest that expression of the GH gene is very low immediately after hatching, and increases dramatically within 6 days.

Original languageEnglish
Pages (from-to)R7
JournalMolecular and Cellular Endocrinology
Volume87
Issue number1-3
DOIs
StatePublished - 1 Jan 1992
Externally publishedYes

Keywords

  • Fish
  • Growth hormone
  • Larva
  • RNA blot hybridization
  • β-Actin

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology

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