Developmental expression of the growth hormone gene in the gilthead sea bream Sparus aurata

Bruria Funkenstein; Amos Tandler; Benzion Cavari

doi:10.1016/0303-7207(92)90249-6

Developmental expression of the growth hormone gene in the gilthead sea bream Sparus aurata

Bruria Funkenstein, Amos Tandler, Benzion Cavari

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Expression of growth hormone (GH) gene during early stages of larval development of the teleost Sparus aurata was determined by Northern blot analysis. Poly(A⁺) RNA was prepared from a pool of larvae collected on different days after hatching. When hybridized to Sparus aurata GH cDNA, GH specific mRNA was first seen on day 6 post-hatching. In contrast, the levels of β-actin mRNA, which was used to normalize for RNA amounts, were already high on the day of hatching. Our results suggest that expression of the GH gene is very low immediately after hatching, and increases dramatically within 6 days.

Original language	English
Pages (from-to)	R7
Journal	Molecular and Cellular Endocrinology
Volume	87
Issue number	1-3
DOIs	https://doi.org/10.1016/0303-7207(92)90249-6
State	Published - 1 Jan 1992
Externally published	Yes

Keywords

Fish
Growth hormone
Larva
RNA blot hybridization
β-Actin

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Endocrinology

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10.1016/0303-7207(92)90249-6

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title = "Developmental expression of the growth hormone gene in the gilthead sea bream Sparus aurata",

abstract = "Expression of growth hormone (GH) gene during early stages of larval development of the teleost Sparus aurata was determined by Northern blot analysis. Poly(A+) RNA was prepared from a pool of larvae collected on different days after hatching. When hybridized to Sparus aurata GH cDNA, GH specific mRNA was first seen on day 6 post-hatching. In contrast, the levels of β-actin mRNA, which was used to normalize for RNA amounts, were already high on the day of hatching. Our results suggest that expression of the GH gene is very low immediately after hatching, and increases dramatically within 6 days.",

keywords = "Fish, Growth hormone, Larva, RNA blot hybridization, β-Actin",

author = "Bruria Funkenstein and Amos Tandler and Benzion Cavari",

note = "Funding Information: We are grateful to Hamutal Krugliak for providing us with Sparus aurata larvae used in this study. We also thank Keren Diskin for skilled editorial assistance. This work was supported in part by the Erna and Victor Hasselblad Foundation and in part by the United States - Israel Binational Agricultural Research and Development Fund (BARD, Project No. IS-1973-91).",

year = "1992",

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doi = "10.1016/0303-7207(92)90249-6",

language = "English",

volume = "87",

pages = "R7",

journal = "Molecular and Cellular Endocrinology",

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TY - JOUR

T1 - Developmental expression of the growth hormone gene in the gilthead sea bream Sparus aurata

AU - Funkenstein, Bruria

AU - Tandler, Amos

AU - Cavari, Benzion

N1 - Funding Information: We are grateful to Hamutal Krugliak for providing us with Sparus aurata larvae used in this study. We also thank Keren Diskin for skilled editorial assistance. This work was supported in part by the Erna and Victor Hasselblad Foundation and in part by the United States - Israel Binational Agricultural Research and Development Fund (BARD, Project No. IS-1973-91).

PY - 1992/1/1

Y1 - 1992/1/1

N2 - Expression of growth hormone (GH) gene during early stages of larval development of the teleost Sparus aurata was determined by Northern blot analysis. Poly(A+) RNA was prepared from a pool of larvae collected on different days after hatching. When hybridized to Sparus aurata GH cDNA, GH specific mRNA was first seen on day 6 post-hatching. In contrast, the levels of β-actin mRNA, which was used to normalize for RNA amounts, were already high on the day of hatching. Our results suggest that expression of the GH gene is very low immediately after hatching, and increases dramatically within 6 days.

AB - Expression of growth hormone (GH) gene during early stages of larval development of the teleost Sparus aurata was determined by Northern blot analysis. Poly(A+) RNA was prepared from a pool of larvae collected on different days after hatching. When hybridized to Sparus aurata GH cDNA, GH specific mRNA was first seen on day 6 post-hatching. In contrast, the levels of β-actin mRNA, which was used to normalize for RNA amounts, were already high on the day of hatching. Our results suggest that expression of the GH gene is very low immediately after hatching, and increases dramatically within 6 days.

KW - Fish

KW - Growth hormone

KW - Larva

KW - RNA blot hybridization

KW - β-Actin

UR - https://www.scopus.com/pages/publications/0026714766

U2 - 10.1016/0303-7207(92)90249-6

DO - 10.1016/0303-7207(92)90249-6

M3 - Article

C2 - 1359982

AN - SCOPUS:0026714766

SN - 0303-7207

VL - 87

SP - R7

JO - Molecular and Cellular Endocrinology

JF - Molecular and Cellular Endocrinology

IS - 1-3

ER -

Developmental expression of the growth hormone gene in the gilthead sea bream Sparus aurata

Abstract

Keywords

ASJC Scopus subject areas

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