Developments in cryo-electron tomography for in situ structural analysis

Anna Dubrovsky; Simona Sorrentino; Jan Harapin; K. Tanuj Sapra; Ohad Medalia

doi:10.1016/j.abb.2015.04.006

Developments in cryo-electron tomography for in situ structural analysis

Anna Dubrovsky, Simona Sorrentino, Jan Harapin, K. Tanuj Sapra, Ohad Medalia

Department of Life Sciences

Research output: Contribution to journal › Review article › peer-review

22 Scopus citations

Abstract

Structural analysis of macromolecular assemblies and their remodeling during physiological processes is instrumental to defining the fundament of cellular and molecular biology. Recent advances in computational and analytical tools for cryo-electron tomography have enabled the study of macromolecular structures in their native environment, providing unprecedented insights into cell function. Moreover, the recent implementation of direct electron detectors has progressed cryo-electron tomography to a stage where it can now be applied to the reconstruction of macromolecular structures at high resolutions. Here, we discuss some of the recent technical developments in cryo-electron tomography to reveal structures of macromolecular complexes in their physiological medium, focusing mainly on eukaryotic cells.

Original language	English
Pages (from-to)	78-85
Number of pages	8
Journal	Archives of Biochemistry and Biophysics
Volume	581
DOIs	https://doi.org/10.1016/j.abb.2015.04.006
State	Published - 17 Aug 2015

Keywords

Cryo-electron tomography
Cryo-focused-ion beam
Direct electron detector
Phase plate

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology

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10.1016/j.abb.2015.04.006

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title = "Developments in cryo-electron tomography for in situ structural analysis",

abstract = "Structural analysis of macromolecular assemblies and their remodeling during physiological processes is instrumental to defining the fundament of cellular and molecular biology. Recent advances in computational and analytical tools for cryo-electron tomography have enabled the study of macromolecular structures in their native environment, providing unprecedented insights into cell function. Moreover, the recent implementation of direct electron detectors has progressed cryo-electron tomography to a stage where it can now be applied to the reconstruction of macromolecular structures at high resolutions. Here, we discuss some of the recent technical developments in cryo-electron tomography to reveal structures of macromolecular complexes in their physiological medium, focusing mainly on eukaryotic cells.",

keywords = "Cryo-electron tomography, Cryo-focused-ion beam, Direct electron detector, Phase plate",

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AU - Dubrovsky, Anna

AU - Sorrentino, Simona

AU - Harapin, Jan

AU - Sapra, K. Tanuj

AU - Medalia, Ohad

PY - 2015/8/17

Y1 - 2015/8/17

N2 - Structural analysis of macromolecular assemblies and their remodeling during physiological processes is instrumental to defining the fundament of cellular and molecular biology. Recent advances in computational and analytical tools for cryo-electron tomography have enabled the study of macromolecular structures in their native environment, providing unprecedented insights into cell function. Moreover, the recent implementation of direct electron detectors has progressed cryo-electron tomography to a stage where it can now be applied to the reconstruction of macromolecular structures at high resolutions. Here, we discuss some of the recent technical developments in cryo-electron tomography to reveal structures of macromolecular complexes in their physiological medium, focusing mainly on eukaryotic cells.

AB - Structural analysis of macromolecular assemblies and their remodeling during physiological processes is instrumental to defining the fundament of cellular and molecular biology. Recent advances in computational and analytical tools for cryo-electron tomography have enabled the study of macromolecular structures in their native environment, providing unprecedented insights into cell function. Moreover, the recent implementation of direct electron detectors has progressed cryo-electron tomography to a stage where it can now be applied to the reconstruction of macromolecular structures at high resolutions. Here, we discuss some of the recent technical developments in cryo-electron tomography to reveal structures of macromolecular complexes in their physiological medium, focusing mainly on eukaryotic cells.

KW - Cryo-electron tomography

KW - Cryo-focused-ion beam

KW - Direct electron detector

KW - Phase plate

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M3 - Review article

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SN - 0003-9861

VL - 581

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JO - Archives of Biochemistry and Biophysics

JF - Archives of Biochemistry and Biophysics

ER -

Developments in cryo-electron tomography for in situ structural analysis

Abstract

Keywords

ASJC Scopus subject areas

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