Developments in cryo-electron tomography for in situ structural analysis

Anna Dubrovsky, Simona Sorrentino, Jan Harapin, K. Tanuj Sapra, Ohad Medalia

Research output: Contribution to journalReview articlepeer-review

21 Scopus citations


Structural analysis of macromolecular assemblies and their remodeling during physiological processes is instrumental to defining the fundament of cellular and molecular biology. Recent advances in computational and analytical tools for cryo-electron tomography have enabled the study of macromolecular structures in their native environment, providing unprecedented insights into cell function. Moreover, the recent implementation of direct electron detectors has progressed cryo-electron tomography to a stage where it can now be applied to the reconstruction of macromolecular structures at high resolutions. Here, we discuss some of the recent technical developments in cryo-electron tomography to reveal structures of macromolecular complexes in their physiological medium, focusing mainly on eukaryotic cells.

Original languageEnglish
Pages (from-to)78-85
Number of pages8
JournalArchives of Biochemistry and Biophysics
StatePublished - 17 Aug 2015


  • Cryo-electron tomography
  • Cryo-focused-ion beam
  • Direct electron detector
  • Phase plate


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