Diazirine based photoaffinity labeling

Luba Dubinsky; Bastiaan P. Krom; Michael M. Meijler

doi:10.1016/j.bmc.2011.06.066

Diazirine based photoaffinity labeling

Luba Dubinsky, Bastiaan P. Krom, Michael M. Meijler

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

321 Scopus citations

Abstract

Diazirines are among the smallest photoreactive groups that form a reactive carbene upon light irradiation. This feature has been widely utilized in photoaffinity labeling to study ligand-receptor, ligand-enzyme and protein-protein interactions, and in the isolation and identification of unknown proteins. This review summarizes recent advances in the use of diazirines in photoaffinity labeling.

Original language	English
Pages (from-to)	554-570
Number of pages	17
Journal	Bioorganic and Medicinal Chemistry
Volume	20
Issue number	2
DOIs	https://doi.org/10.1016/j.bmc.2011.06.066
State	Published - 15 Jan 2012

Keywords

Chemical proteomics
Diazirine
Photoaffinity labeling
Photochemistry
Protein profiling

ASJC Scopus subject areas

Biochemistry
Molecular Medicine
Molecular Biology
Pharmaceutical Science
Drug Discovery
Clinical Biochemistry
Organic Chemistry

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.bmc.2011.06.066

Cite this

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title = "Diazirine based photoaffinity labeling",

abstract = "Diazirines are among the smallest photoreactive groups that form a reactive carbene upon light irradiation. This feature has been widely utilized in photoaffinity labeling to study ligand-receptor, ligand-enzyme and protein-protein interactions, and in the isolation and identification of unknown proteins. This review summarizes recent advances in the use of diazirines in photoaffinity labeling.",

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author = "Luba Dubinsky and Krom, {Bastiaan P.} and Meijler, {Michael M.}",

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AU - Dubinsky, Luba

AU - Krom, Bastiaan P.

AU - Meijler, Michael M.

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N2 - Diazirines are among the smallest photoreactive groups that form a reactive carbene upon light irradiation. This feature has been widely utilized in photoaffinity labeling to study ligand-receptor, ligand-enzyme and protein-protein interactions, and in the isolation and identification of unknown proteins. This review summarizes recent advances in the use of diazirines in photoaffinity labeling.

AB - Diazirines are among the smallest photoreactive groups that form a reactive carbene upon light irradiation. This feature has been widely utilized in photoaffinity labeling to study ligand-receptor, ligand-enzyme and protein-protein interactions, and in the isolation and identification of unknown proteins. This review summarizes recent advances in the use of diazirines in photoaffinity labeling.

KW - Chemical proteomics

KW - Diazirine

KW - Photoaffinity labeling

KW - Photochemistry

KW - Protein profiling

UR - http://www.scopus.com/inward/record.url?scp=84855816511&partnerID=8YFLogxK

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DO - 10.1016/j.bmc.2011.06.066

M3 - Article

AN - SCOPUS:84855816511

SN - 0968-0896

VL - 20

SP - 554

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JO - Bioorganic and Medicinal Chemistry

JF - Bioorganic and Medicinal Chemistry

IS - 2

ER -

Diazirine based photoaffinity labeling

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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