Differences in metabolite levels upon differentiation of intact neuroblastoma × glioma cells observed by proton NMR spectroscopy

Gil Navon; Howard Burrows; Jack S. Cohen

doi:10.1016/0014-5793(83)80780-7

Differences in metabolite levels upon differentiation of intact neuroblastoma × glioma cells observed by proton NMR spectroscopy

Gil Navon, Howard Burrows, Jack S. Cohen

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

Proton NMR spectroscopy was used to study the effect of differentiation with prostaglandin E₁ and theophylline on intact hybrid neuroblastoma × glioma cells. The standard proton NMR method showed more resolvable signals than the spin echo NMR spectra. The differentiated cells were found to contain significantly higher levels of glutamine than the undifferentiated precursors. Observations on cell extracts confirmed these results.

Original language	English
Pages (from-to)	320-323
Number of pages	4
Journal	FEBS Letters
Volume	162
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(83)80780-7
State	Published - 17 Oct 1983
Externally published	Yes

Keywords

Differentiation
Glutamine
Neuroblastoma × glioma cell
Prostaglandin E
Proton NMR vs spin-echo
Theophylline

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/0014-5793(83)80780-7

Cite this

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title = "Differences in metabolite levels upon differentiation of intact neuroblastoma × glioma cells observed by proton NMR spectroscopy",

abstract = "Proton NMR spectroscopy was used to study the effect of differentiation with prostaglandin E1 and theophylline on intact hybrid neuroblastoma × glioma cells. The standard proton NMR method showed more resolvable signals than the spin echo NMR spectra. The differentiated cells were found to contain significantly higher levels of glutamine than the undifferentiated precursors. Observations on cell extracts confirmed these results.",

keywords = "Differentiation, Glutamine, Neuroblastoma × glioma cell, Prostaglandin E, Proton NMR vs spin-echo, Theophylline",

author = "Gil Navon and Howard Burrows and Cohen, {Jack S.}",

year = "1983",

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journal = "FEBS Letters",

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T1 - Differences in metabolite levels upon differentiation of intact neuroblastoma × glioma cells observed by proton NMR spectroscopy

AU - Navon, Gil

AU - Burrows, Howard

AU - Cohen, Jack S.

PY - 1983/10/17

Y1 - 1983/10/17

N2 - Proton NMR spectroscopy was used to study the effect of differentiation with prostaglandin E1 and theophylline on intact hybrid neuroblastoma × glioma cells. The standard proton NMR method showed more resolvable signals than the spin echo NMR spectra. The differentiated cells were found to contain significantly higher levels of glutamine than the undifferentiated precursors. Observations on cell extracts confirmed these results.

AB - Proton NMR spectroscopy was used to study the effect of differentiation with prostaglandin E1 and theophylline on intact hybrid neuroblastoma × glioma cells. The standard proton NMR method showed more resolvable signals than the spin echo NMR spectra. The differentiated cells were found to contain significantly higher levels of glutamine than the undifferentiated precursors. Observations on cell extracts confirmed these results.

KW - Differentiation

KW - Glutamine

KW - Neuroblastoma × glioma cell

KW - Prostaglandin E

KW - Proton NMR vs spin-echo

KW - Theophylline

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U2 - 10.1016/0014-5793(83)80780-7

DO - 10.1016/0014-5793(83)80780-7

M3 - Article

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AN - SCOPUS:0020559086

SN - 0014-5793

VL - 162

SP - 320

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JO - FEBS Letters

JF - FEBS Letters

IS - 2

ER -

Differences in metabolite levels upon differentiation of intact neuroblastoma × glioma cells observed by proton NMR spectroscopy

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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