Different effects of magnesium sulfate and angiotensin II on the capacity of the fetal and maternal compartments of normal human placenta to secrete TNF-α and IL-6

Gershon Holcberg, Alaa Amash, Olga Sapir, Mordechai Hallak, Eyal Sheiner, Doron Ducler, Miriam Katz, Mahmoud Huleihel

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Three different protocols were carried out to evaluate the effect of magnesium sulfate (MgSO4) on the capacity of the normal human placenta to secrete TNF-α and IL-6 in presence and absence of angiotensin II (AII). Ten placentas were perfused with MgSO4 (6-7 mg%) or medium in the absence or presence of AII. Perfusate samples from fetal and maternal sites were collected and examined for IL-6 and TNF-α by ELISA. The maternal site of placentas exposed to AII showed only basal levels of TNF-α. Exposure of the placentas to MgSO4 resulted in significant increase in TNF-α and IL-6 in the maternal site (p < 0.05). However, the effect of MgSO4 was significantly attenuated by AII injected in presence of MgSO4. TNF-α and IL-6 levels in the maternal site significantly decreased (p < 0.05). The fetal site of the placentas exposed to MgSO4 or AII separately showed only basal levels of TNF-α and IL-6. However, TNF-α and IL-6 levels were significantly higher after injection of AII in the presence of MgSO4 compared to TNF-α levels in the fetal site of placentas exposed to AII alone (p < 0.05) or MgSO4 alone (p < 0.01). MgSO4 induced the ability of the placental maternal site to secrete TNF-α and IL-6. In the presence of both MgSO4 and AII, the effect of MgSO 4 on the maternal site was significantly reduced. However, in the fetal site, MgSO4 alone had no significant effect on TNF-α and IL-6 levels although, in presence of AII and MgSO4, there was a significant increase in TNF-α and IL-6 levels. Elevation of TNF-α and IL-6 in the fetal compartment, which may affect fetal brain growth and development and placental function should be considered before administration of MgSO4 during pregnancy.

Original languageEnglish
Pages (from-to)115-125
Number of pages11
JournalJournal of Reproductive Immunology
Volume69
Issue number2
DOIs
StatePublished - 1 Mar 2006

Keywords

  • Angiotensin II
  • IL-6
  • Inflammatory cytokines
  • MgSO
  • Placenta
  • Tumor necrosis factor

Fingerprint

Dive into the research topics of 'Different effects of magnesium sulfate and angiotensin II on the capacity of the fetal and maternal compartments of normal human placenta to secrete TNF-α and IL-6'. Together they form a unique fingerprint.

Cite this