Differential cellular responses to adhesive interactions with galectin-8- And fibronectin-coated substrates

Wenhong Li, Ana Sancho, Wen Lu Chung, Yaron Vinik, Jürgen Groll, Yehiel Zick, Ohad Medalia, Alexander D. Bershadsky, Benjamin Geiger

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


The mechanisms underlying the cellular response to extracellular matrices (ECMs) that consist of multiple adhesive ligands are still poorly understood. Here, we address this topic by monitoring specific cellular responses to two different extracellular adhesion molecules - the main integrin ligand fibronectin and galectin-8, a lectin that binds β-galactoside residues − as well as to mixtures of the two proteins. Compared with cell spreading on fibronectin, cell spreading on galectin-8-coated substrates resulted in increased projected cell area, more-pronounced extension of filopodia and, yet, the inability to form focal adhesions and stress fibers. These differences can be partially reversed by experimental manipulations of small G-proteins of the Rho family and their downstream targets, such as formins, the Arp2/3 complex and Rho kinase. We also show that the physical adhesion of cells to galectin-8 was stronger than adhesion to fibronectin. Notably, galectin-8 and fibronectin differently regulate cell spreading and focal adhesion formation, yet act synergistically to upregulate the number and length of filopodia. The physiological significance of the coherent cellular response to a molecularly complex matrix is discussed.

Original languageEnglish
Article numberjcs252221
JournalJournal of Cell Science
Issue number8
StatePublished - 1 Apr 2021
Externally publishedYes


  • Extracellular matrix
  • Filopodia
  • Focal adhesions
  • Lamellipodia
  • Myosin-II
  • Rho GTPases

ASJC Scopus subject areas

  • Cell Biology


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