Differential diagnosis of hepatocellular carcinoma from metastatic tumors in the liver using microRNA expression

Iris Barshack, Eti Meiri, Shai Rosenwald, Danit Lebanony, Meital Bronfeld, Sarit Aviel-Ronen, Kinneret Rosenblatt, Sylvie Polak-Charcon, Ilit Leizerman, Meital Ezagouri, Merav Zepeniuk, Norberto Shabes, Lahav Cohen, Sarit Tabak, Dalia Cohen, Zvi Bentwich, Nitzan Rosenfeld

Research output: Contribution to journalArticlepeer-review

39 Scopus citations

Abstract

Distinguishing hepatocellular carcinoma from metastatic tumors in the liver is of great practical importance, with significant therapeutic and prognostic implications. This differential diagnosis can be difficult because metastatic cancers in the liver, especially adenocarcinomas, may mimic the morphology and immunoexpression of hepatocellular carcinoma. Biomarkers that are specifically expressed in either hepatocellular carcinoma or metastatic adenocarcinoma can therefore be useful diagnostic tools. To find such biomarkers, we studied microRNA expression in 144 tumor samples using custom microarrays. Hsa-miR-141 and hsa-miR-200c, microRNAs that promote epithelial phenotypes, had significantly higher levels in non-hepatic epithelial tumors. In contrast, endothelial-associated hsa-miR-126 showed higher expression levels in hepatocellular carcinomas. Combinations of these microRNAs accurately identified primary hepatocellular carcinoma from metastatic adenocarcinoma in the liver. These findings were validated using quantitative real-time PCR to measure microRNA expression in additional samples. Thus, the tissue-specific expression patterns of microRNAs make them useful biomarkers for the diagnosis of liver malignancies.

Original languageEnglish
Pages (from-to)1355-1362
Number of pages8
JournalInternational Journal of Biochemistry and Cell Biology
Volume42
Issue number8
DOIs
StatePublished - 1 Aug 2010
Externally publishedYes

Keywords

  • HCC
  • MiRNA
  • Molecular diagnostics
  • Pathology

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