Differential distributions of two adducin-like protein isoforms in the Drosophila ovary and early embryo

Michèle Zaccai, Howard D. Lipshitz

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84 Scopus citations


Adducin is a cytoskeletal protein that can function in vitro to bundle F-actin and to control the assembly of the F-actin/spectrin cytoskeletal network. The Drosophila Adducin-like (Add) locus (also referred to as hu-li tai shao (hts)) encodes a family of proteins of which several are homologous to mammalian adducin (Ding et al., Proc. Natl. Acad. Sci. USA 90, 2512-16, 1993; Yue & Spradling, Genes Dev. 6, 2443-54, 1992). We report the identification of two novel adducin isoforms: a 95 × 103 Mr form (ADD-95) and an 87 × 103 Mr form (ADO-87). We present a detailed analysis of the distribution patterns of ADD-95 and ADD-87 during oogenesis and embryogenesis. The isoforms are co-expressed in several cell- and tissue-types; however, only ADD-87 is present in mid- to late-stage oocytes. ADD-87 is present throughout the oocyte cortex at stages 9 and 10 of oogenesis but is detectable only at the anterior pole from stage 11 onward, correlated with localisation of Add-hts mRNA first to the cortex and then to the anterior pole of the oocyte. ADD-87 co-localises with F-actin and spectrin in the cortex of the oocyte through stage 10 of oogenesis, consistent with a possible role in cytoskeletal assembly or function predicted by mammalian studies.

Original languageEnglish
Pages (from-to)159-166
Number of pages8
Issue number2
StatePublished - 1 Jan 1996


  • Adducin
  • Cytoskeleton
  • Drosophila

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology


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