Differential distributions of two adducin-like protein isoforms in the Drosophila ovary and early embryo

Michèle Zaccai; Howard D. Lipshitz

doi:10.1017/S096719940000304X

Differential distributions of two adducin-like protein isoforms in the Drosophila ovary and early embryo

Michèle Zaccai, Howard D. Lipshitz

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

84 Scopus citations

Abstract

Adducin is a cytoskeletal protein that can function in vitro to bundle F-actin and to control the assembly of the F-actin/spectrin cytoskeletal network. The Drosophila Adducin-like (Add) locus (also referred to as hu-li tai shao (hts)) encodes a family of proteins of which several are homologous to mammalian adducin (Ding et al., Proc. Natl. Acad. Sci. USA 90, 2512-16, 1993; Yue & Spradling, Genes Dev. 6, 2443-54, 1992). We report the identification of two novel adducin isoforms: a 95 × 10³ M_r form (ADD-95) and an 87 × 10³ M_r form (ADO-87). We present a detailed analysis of the distribution patterns of ADD-95 and ADD-87 during oogenesis and embryogenesis. The isoforms are co-expressed in several cell- and tissue-types; however, only ADD-87 is present in mid- to late-stage oocytes. ADD-87 is present throughout the oocyte cortex at stages 9 and 10 of oogenesis but is detectable only at the anterior pole from stage 11 onward, correlated with localisation of Add-hts mRNA first to the cortex and then to the anterior pole of the oocyte. ADD-87 co-localises with F-actin and spectrin in the cortex of the oocyte through stage 10 of oogenesis, consistent with a possible role in cytoskeletal assembly or function predicted by mammalian studies.

Original language	English
Pages (from-to)	159-166
Number of pages	8
Journal	Zygote
Volume	4
Issue number	2
DOIs	https://doi.org/10.1017/S096719940000304X
State	Published - 1 Jan 1996

Keywords

Adducin
Cytoskeleton
Drosophila

ASJC Scopus subject areas

Developmental Biology
Cell Biology

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10.1017/S096719940000304X

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@article{7b3e4cd21d46436eb0879b1ce551adab,

title = "Differential distributions of two adducin-like protein isoforms in the Drosophila ovary and early embryo",

abstract = "Adducin is a cytoskeletal protein that can function in vitro to bundle F-actin and to control the assembly of the F-actin/spectrin cytoskeletal network. The Drosophila Adducin-like (Add) locus (also referred to as hu-li tai shao (hts)) encodes a family of proteins of which several are homologous to mammalian adducin (Ding et al., Proc. Natl. Acad. Sci. USA 90, 2512-16, 1993; Yue & Spradling, Genes Dev. 6, 2443-54, 1992). We report the identification of two novel adducin isoforms: a 95 × 103 Mr form (ADD-95) and an 87 × 103 Mr form (ADO-87). We present a detailed analysis of the distribution patterns of ADD-95 and ADD-87 during oogenesis and embryogenesis. The isoforms are co-expressed in several cell- and tissue-types; however, only ADD-87 is present in mid- to late-stage oocytes. ADD-87 is present throughout the oocyte cortex at stages 9 and 10 of oogenesis but is detectable only at the anterior pole from stage 11 onward, correlated with localisation of Add-hts mRNA first to the cortex and then to the anterior pole of the oocyte. ADD-87 co-localises with F-actin and spectrin in the cortex of the oocyte through stage 10 of oogenesis, consistent with a possible role in cytoskeletal assembly or function predicted by mammalian studies.",

keywords = "Adducin, Cytoskeleton, Drosophila",

author = "Mich{\`e}le Zaccai and Lipshitz, {Howard D.}",

note = "Funding Information: We are grateful to Dr Daniel Kiehart (Duke University) for providing the anti-spectrin antibodies; Dr Ker-Hua (Susan) Ou for producing the anti-ADD antibodies at the Caltech Monoclonal Antibody Facility; Dr Man Lun (Richard) Yip and William Fisher for expressing and purifying GST-ADD fusion protein; Drs Scott Fraser and Gary Belford for instruction in the use of the confocal microscope at the Biological Imaging Facility of Caltech's Beckman Institute; Dr Elliot Meyerowitz for the use of his computer and slide scanner; Dr Susan Halsell for help with RNA tissue in situ hybridisation and for her perceptive comments on the Drosophila cytoskeleton; Dr Susan Celniker for useful suggestions on immunoblorting procedures; Dr Susan Celniker, Dr Michele Lamka, Peter Becker and Kellie Whittaker for providing insightful criticisms of the manuscript. M.Z. was supported in part by an Alcott Postdoctoral Fellowship from the Biology Division at Caltech. This research was supported with funds provided to H.D.L. by the Gustavus and Louise Pfeifer Research Foundation and Millard and Muriel Jacobs.",

year = "1996",

month = jan,

day = "1",

doi = "10.1017/S096719940000304X",

language = "English",

volume = "4",

pages = "159--166",

journal = "Zygote",

issn = "0967-1994",

publisher = "Cambridge University Press",

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TY - JOUR

T1 - Differential distributions of two adducin-like protein isoforms in the Drosophila ovary and early embryo

AU - Zaccai, Michèle

AU - Lipshitz, Howard D.

N1 - Funding Information: We are grateful to Dr Daniel Kiehart (Duke University) for providing the anti-spectrin antibodies; Dr Ker-Hua (Susan) Ou for producing the anti-ADD antibodies at the Caltech Monoclonal Antibody Facility; Dr Man Lun (Richard) Yip and William Fisher for expressing and purifying GST-ADD fusion protein; Drs Scott Fraser and Gary Belford for instruction in the use of the confocal microscope at the Biological Imaging Facility of Caltech's Beckman Institute; Dr Elliot Meyerowitz for the use of his computer and slide scanner; Dr Susan Halsell for help with RNA tissue in situ hybridisation and for her perceptive comments on the Drosophila cytoskeleton; Dr Susan Celniker for useful suggestions on immunoblorting procedures; Dr Susan Celniker, Dr Michele Lamka, Peter Becker and Kellie Whittaker for providing insightful criticisms of the manuscript. M.Z. was supported in part by an Alcott Postdoctoral Fellowship from the Biology Division at Caltech. This research was supported with funds provided to H.D.L. by the Gustavus and Louise Pfeifer Research Foundation and Millard and Muriel Jacobs.

PY - 1996/1/1

Y1 - 1996/1/1

N2 - Adducin is a cytoskeletal protein that can function in vitro to bundle F-actin and to control the assembly of the F-actin/spectrin cytoskeletal network. The Drosophila Adducin-like (Add) locus (also referred to as hu-li tai shao (hts)) encodes a family of proteins of which several are homologous to mammalian adducin (Ding et al., Proc. Natl. Acad. Sci. USA 90, 2512-16, 1993; Yue & Spradling, Genes Dev. 6, 2443-54, 1992). We report the identification of two novel adducin isoforms: a 95 × 103 Mr form (ADD-95) and an 87 × 103 Mr form (ADO-87). We present a detailed analysis of the distribution patterns of ADD-95 and ADD-87 during oogenesis and embryogenesis. The isoforms are co-expressed in several cell- and tissue-types; however, only ADD-87 is present in mid- to late-stage oocytes. ADD-87 is present throughout the oocyte cortex at stages 9 and 10 of oogenesis but is detectable only at the anterior pole from stage 11 onward, correlated with localisation of Add-hts mRNA first to the cortex and then to the anterior pole of the oocyte. ADD-87 co-localises with F-actin and spectrin in the cortex of the oocyte through stage 10 of oogenesis, consistent with a possible role in cytoskeletal assembly or function predicted by mammalian studies.

AB - Adducin is a cytoskeletal protein that can function in vitro to bundle F-actin and to control the assembly of the F-actin/spectrin cytoskeletal network. The Drosophila Adducin-like (Add) locus (also referred to as hu-li tai shao (hts)) encodes a family of proteins of which several are homologous to mammalian adducin (Ding et al., Proc. Natl. Acad. Sci. USA 90, 2512-16, 1993; Yue & Spradling, Genes Dev. 6, 2443-54, 1992). We report the identification of two novel adducin isoforms: a 95 × 103 Mr form (ADD-95) and an 87 × 103 Mr form (ADO-87). We present a detailed analysis of the distribution patterns of ADD-95 and ADD-87 during oogenesis and embryogenesis. The isoforms are co-expressed in several cell- and tissue-types; however, only ADD-87 is present in mid- to late-stage oocytes. ADD-87 is present throughout the oocyte cortex at stages 9 and 10 of oogenesis but is detectable only at the anterior pole from stage 11 onward, correlated with localisation of Add-hts mRNA first to the cortex and then to the anterior pole of the oocyte. ADD-87 co-localises with F-actin and spectrin in the cortex of the oocyte through stage 10 of oogenesis, consistent with a possible role in cytoskeletal assembly or function predicted by mammalian studies.

KW - Adducin

KW - Cytoskeleton

KW - Drosophila

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U2 - 10.1017/S096719940000304X

DO - 10.1017/S096719940000304X

M3 - Article

AN - SCOPUS:0030133837

SN - 0967-1994

VL - 4

SP - 159

EP - 166

JO - Zygote

JF - Zygote

IS - 2

ER -

Differential distributions of two adducin-like protein isoforms in the Drosophila ovary and early embryo

Abstract

Keywords

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