Differential expression of microRNAs with progression of gestation and inflammation in the human chorioamniotic membranes

Daniel Montenegro; Roberto Romero; Beth L. Pineles; Adi L. Tarca; Yeon Mee Kim; Sorin Draghici; Juan Pedro Kusanovic; Jung Sun Kim; Offer Erez; Shali Mazaki-Tovi; Sonia Hassan; Jimmy Espinoza; Chong Jai Kim

doi:10.1016/j.ajog.2007.06.027

Differential expression of microRNAs with progression of gestation and inflammation in the human chorioamniotic membranes

Daniel Montenegro, Roberto Romero, Beth L. Pineles, Adi L. Tarca, Yeon Mee Kim, Sorin Draghici, Juan Pedro Kusanovic, Jung Sun Kim, Offer Erez, Shali Mazaki-Tovi, Sonia Hassan, Jimmy Espinoza, Chong Jai Kim

Research output: Contribution to journal › Article › peer-review

50 Scopus citations

Abstract

Objective: The aim of this study was to identify differential expression of microRNAs (miRNAs) in chorioamniotic membranes with advancing gestation, labor, and inflammation. Study Design: Expression profiles of 157 miRNAs in the chorioamniotic membranes were obtained from patients in the following groups: 1) term not in labor (n = 10); 2) term in labor (n = 10); 3) preterm labor with histologic chorioamionitis (n = 9); and 4) without histologic chorioamnionitis (n = 10). Results: More than 95% of the miRNAs screened were expressed. Gestational age-dependent changes in expression were observed for 13 miRNAs. No differences in miRNA expression were observed between women without labor and women in labor. Membranes with chorioamnionitis displayed increased expression of miR-223 and miR-338. Gene Ontology analysis of genes targeted by differentially expressed miRNAs revealed enrichment for specific biological process categories. Conclusion: Chorioamniotic membranes with advancing gestational age and chorioamnionitis are associated with the differential expression of a subset of miRNAs.

Original language	English
Pages (from-to)	289.e1-289.e6
Journal	American Journal of Obstetrics and Gynecology
Volume	197
Issue number	3
DOIs	https://doi.org/10.1016/j.ajog.2007.06.027
State	Published - 1 Jan 2007
Externally published	Yes

Keywords

chorioamniotic membranes
gestation
inflammation
labor
microRNA
real-time qRT-PCR

ASJC Scopus subject areas

Obstetrics and Gynecology

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10.1016/j.ajog.2007.06.027

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Montenegro, D., Romero, R., Pineles, B. L., Tarca, A. L., Kim, Y. M., Draghici, S., Kusanovic, J. P., Kim, J. S., Erez, O., Mazaki-Tovi, S., Hassan, S., Espinoza, J., & Kim, C. J. (2007). Differential expression of microRNAs with progression of gestation and inflammation in the human chorioamniotic membranes. American Journal of Obstetrics and Gynecology, 197(3), 289.e1-289.e6. https://doi.org/10.1016/j.ajog.2007.06.027

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title = "Differential expression of microRNAs with progression of gestation and inflammation in the human chorioamniotic membranes",

abstract = "Objective: The aim of this study was to identify differential expression of microRNAs (miRNAs) in chorioamniotic membranes with advancing gestation, labor, and inflammation. Study Design: Expression profiles of 157 miRNAs in the chorioamniotic membranes were obtained from patients in the following groups: 1) term not in labor (n = 10); 2) term in labor (n = 10); 3) preterm labor with histologic chorioamionitis (n = 9); and 4) without histologic chorioamnionitis (n = 10). Results: More than 95% of the miRNAs screened were expressed. Gestational age-dependent changes in expression were observed for 13 miRNAs. No differences in miRNA expression were observed between women without labor and women in labor. Membranes with chorioamnionitis displayed increased expression of miR-223 and miR-338. Gene Ontology analysis of genes targeted by differentially expressed miRNAs revealed enrichment for specific biological process categories. Conclusion: Chorioamniotic membranes with advancing gestational age and chorioamnionitis are associated with the differential expression of a subset of miRNAs.",

keywords = "chorioamniotic membranes, gestation, inflammation, labor, microRNA, real-time qRT-PCR",

author = "Daniel Montenegro and Roberto Romero and Pineles, {Beth L.} and Tarca, {Adi L.} and Kim, {Yeon Mee} and Sorin Draghici and Kusanovic, {Juan Pedro} and Kim, {Jung Sun} and Offer Erez and Shali Mazaki-Tovi and Sonia Hassan and Jimmy Espinoza and Kim, {Chong Jai}",

note = "Funding Information: Supported by the Intramural Research Program of the National Institute of Child Health and Human Development, NIH, DHHS. ",

year = "2007",

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doi = "10.1016/j.ajog.2007.06.027",

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Montenegro, D, Romero, R, Pineles, BL, Tarca, AL, Kim, YM, Draghici, S, Kusanovic, JP, Kim, JS, Erez, O, Mazaki-Tovi, S, Hassan, S, Espinoza, J & Kim, CJ 2007, 'Differential expression of microRNAs with progression of gestation and inflammation in the human chorioamniotic membranes', American Journal of Obstetrics and Gynecology, vol. 197, no. 3, pp. 289.e1-289.e6. https://doi.org/10.1016/j.ajog.2007.06.027

TY - JOUR

T1 - Differential expression of microRNAs with progression of gestation and inflammation in the human chorioamniotic membranes

AU - Montenegro, Daniel

AU - Romero, Roberto

AU - Pineles, Beth L.

AU - Tarca, Adi L.

AU - Kim, Yeon Mee

AU - Draghici, Sorin

AU - Kusanovic, Juan Pedro

AU - Kim, Jung Sun

AU - Erez, Offer

AU - Mazaki-Tovi, Shali

AU - Hassan, Sonia

AU - Espinoza, Jimmy

AU - Kim, Chong Jai

N1 - Funding Information: Supported by the Intramural Research Program of the National Institute of Child Health and Human Development, NIH, DHHS.

PY - 2007/1/1

Y1 - 2007/1/1

N2 - Objective: The aim of this study was to identify differential expression of microRNAs (miRNAs) in chorioamniotic membranes with advancing gestation, labor, and inflammation. Study Design: Expression profiles of 157 miRNAs in the chorioamniotic membranes were obtained from patients in the following groups: 1) term not in labor (n = 10); 2) term in labor (n = 10); 3) preterm labor with histologic chorioamionitis (n = 9); and 4) without histologic chorioamnionitis (n = 10). Results: More than 95% of the miRNAs screened were expressed. Gestational age-dependent changes in expression were observed for 13 miRNAs. No differences in miRNA expression were observed between women without labor and women in labor. Membranes with chorioamnionitis displayed increased expression of miR-223 and miR-338. Gene Ontology analysis of genes targeted by differentially expressed miRNAs revealed enrichment for specific biological process categories. Conclusion: Chorioamniotic membranes with advancing gestational age and chorioamnionitis are associated with the differential expression of a subset of miRNAs.

AB - Objective: The aim of this study was to identify differential expression of microRNAs (miRNAs) in chorioamniotic membranes with advancing gestation, labor, and inflammation. Study Design: Expression profiles of 157 miRNAs in the chorioamniotic membranes were obtained from patients in the following groups: 1) term not in labor (n = 10); 2) term in labor (n = 10); 3) preterm labor with histologic chorioamionitis (n = 9); and 4) without histologic chorioamnionitis (n = 10). Results: More than 95% of the miRNAs screened were expressed. Gestational age-dependent changes in expression were observed for 13 miRNAs. No differences in miRNA expression were observed between women without labor and women in labor. Membranes with chorioamnionitis displayed increased expression of miR-223 and miR-338. Gene Ontology analysis of genes targeted by differentially expressed miRNAs revealed enrichment for specific biological process categories. Conclusion: Chorioamniotic membranes with advancing gestational age and chorioamnionitis are associated with the differential expression of a subset of miRNAs.

KW - chorioamniotic membranes

KW - gestation

KW - inflammation

KW - labor

KW - microRNA

KW - real-time qRT-PCR

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U2 - 10.1016/j.ajog.2007.06.027

DO - 10.1016/j.ajog.2007.06.027

M3 - Article

AN - SCOPUS:34548476553

SN - 0002-9378

VL - 197

SP - 289.e1-289.e6

JO - American Journal of Obstetrics and Gynecology

JF - American Journal of Obstetrics and Gynecology

IS - 3

ER -

Differential expression of microRNAs with progression of gestation and inflammation in the human chorioamniotic membranes

Abstract

Keywords

ASJC Scopus subject areas

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