Differential expression of the H-2D(k) MHC class-I antigen and tissue inhibitor of metalloproteinases in metastatic and nonmetastatic T-10 fibrosarcoma cells

I. L. Har-Vardi, J. Gopas, B. Rager-Zisman, G. J. Hammerling, S. Segal, M. Aboud

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3 Scopus citations

Abstract

In previous studies, we have demonstrated that the highly metastatic IE-7 cell clone, derived from the T-10 fibrosarcoma, expressed both the H-2D(k) and H-2Db genes, whereas a nonmetastatic IC-9 clone, derived from the same tumor, expressed only H-2Db, suggesting that the H-2D(k) product might be involved in the metastatic phenotype. To substantiate this notion, IC-9 cells were transfected with an H-2D(k)-expressing vector. Although all of the 4 randomly selected transfectant subclones elicited high H-2D(k) expression, only one was as metastatic as IE-7 cells. This metastatic transfectant resembled IE-7 cells also in its inability to evoke CTL response in syngeneic mice, whereas the other transfectants were quite competent in this respect. It thus appears that the H-2D(k) product may contribute to the metastatic phenotype provided that it is immunogenically abnormal. In addition, the present study provides evidence to suggest that lack of production of the tissue inhibitor of metalloproteinases TIMP-1/TIMP-2 is another important determinant in the metastatic phenotype of these cells.

Original languageEnglish
Pages (from-to)301-308
Number of pages8
JournalInvasion and Metastasis
Volume12
Issue number5-6
StatePublished - 1 Dec 1992

ASJC Scopus subject areas

  • Cancer Research

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