TY - JOUR
T1 - Differential inhibition of cellular RNA by photosensitized trioxalen
AU - Shames, Benny
AU - Kalma, Sarit
AU - Heimer, Yair M.
AU - Tal, Jacov
N1 - Funding Information:
ACKNOWLEDGEMENTS We thank D1na Ron for helpful discussions and Rina Eren for technical assistance. This work was supported by grants from the Israel Cancer Society and the U.S.-Israel Binational Science Foundation (to J.T.).
PY - 1983/9/24
Y1 - 1983/9/24
N2 - Treatment of mouse cell cultures with 5 × 10-6;M trioxalen (4,5′,8-trimethylpsoralen, or TMP) followed by long wavelength ("near") UV-irradiation (NUV) resulted 1n immediate cessation of most of the cellular transcriptional activity. The effective inhibitory TMP concentrations for ribosomal RNA were lower than those affecting poly RNA containing RNA. Low molecular RNA species were only partially inhibited. Side effects, commonly caused by other drugs, were not detected. These properties, and the simplicity of their use, make psoralens attractive Inhibitors of RNA biosynthesis.
AB - Treatment of mouse cell cultures with 5 × 10-6;M trioxalen (4,5′,8-trimethylpsoralen, or TMP) followed by long wavelength ("near") UV-irradiation (NUV) resulted 1n immediate cessation of most of the cellular transcriptional activity. The effective inhibitory TMP concentrations for ribosomal RNA were lower than those affecting poly RNA containing RNA. Low molecular RNA species were only partially inhibited. Side effects, commonly caused by other drugs, were not detected. These properties, and the simplicity of their use, make psoralens attractive Inhibitors of RNA biosynthesis.
UR - http://www.scopus.com/inward/record.url?scp=0021111307&partnerID=8YFLogxK
U2 - 10.1093/nar/11.18.6453
DO - 10.1093/nar/11.18.6453
M3 - Article
AN - SCOPUS:0021111307
SN - 0305-1048
VL - 11
SP - 6453
EP - 6464
JO - Nucleic Acids Research
JF - Nucleic Acids Research
IS - 18
ER -