Differential response to single and combined salt and heat stresses: Impact on accumulation of proteins and metabolites in dead pericarps of Brassica juncea

Jeevan R. Singiri, Bupur Swetha, Noga Sikron‐persi, Gideon Grafi

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Dead organs enclosing embryos, such as seed coats and pericarps, are emerging as important maternally‐derived components of the dispersal unit that affect seed performance and fate. In the face of climate change and increased incidents of heatwaves, we sought to investigate the effect of salinity (S), short episodes of high temperature (HS), and combination of S + HS (SHS), at the reproductive phase, on the properties of dead pericarps of Brassica juncea. Proteome and metabolome analyses revealed multiple proteins and metabolites stored in dead pericarps whose levels and composition were altered under single and combined stress conditions. The protein profile of SHS showed a higher correlation with salt than with HS indicating the dominant effect of salt over heat stress. On the other hand, the analysis of metabolites showed that the profile of SHS has better correlation with HS than with salt. The integration of metabolic and proteomic data showed that changes in TCA cycle intermediates and certain amino acids (e.g., proline) under salt treatments (S and SHS) are highly correlated with changes in proteins involved in their biosynthetic pathways. Thus, accumulation of proteins and metabolites in dead pericarps is differently affected by single and combination of salt and heat stresses. Salinity appears to dominate plant response to combined stresses at the protein level, while heat appears to be the major factor affecting metabolite accumulation in dead pericarps.

Original languageEnglish
Article number7076
JournalInternational Journal of Molecular Sciences
Volume22
Issue number13
DOIs
StatePublished - 1 Jul 2021

Keywords

  • Brassica juncea
  • Dead pericarps
  • Phytohormones
  • Reproductive phase
  • Salinity
  • Seed abortion
  • Short episodes of high temperature
  • Stress response

Fingerprint

Dive into the research topics of 'Differential response to single and combined salt and heat stresses: Impact on accumulation of proteins and metabolites in dead pericarps of Brassica juncea'. Together they form a unique fingerprint.

Cite this