Differential signaling patterns of stimulated bone marrow-derived dendritic cells under α1-antitrypsin-enriched conditions

Eyal Ozeri, Peleg Rider, Shoham Rigbi, Galit Shahaf, Iulia I. Nita, Israel Sekler, Eli C. Lewis, Ronen Schuster

Research output: Contribution to journalArticlepeer-review

Abstract

Dendritic cells (DCs) mature upon an inflammatory trigger. However, an inflammatory trigger can lead to a semi-mature phenotype, allowing DCs to evoke tolerance and expedite the resolution of inflammation. This duality likely involves context-dependent modulation of inflammatory signaling. Human α1-antitrypsin (hAAT) promotes semimature DCs. We examined changes in a wide spectrum of signaling cascades in stimulated murine bone marrow-derived cells with hAAT. Upon stimulation by IL-1β+IFNγ, hAAT-treated cells depicted an attenuated calcium flux. Disrupting PKA or NF-κB pathways revoked only some hAAT-mediated outcomes. hAAT-treated cells exhibited a distict pattern of kinase phosphorylation. hAAT-mediated increase in Treg cells in-vitro required intact inflammatory signaling pathways. Taken together, hAAT appears to require a stimulated microenvironment to promote inflammatory resolution, setting it aside from classical anti-inflammatory agents. Further studies are required to identify the specific molecules targeted by hAAT that mediate these and other outcomes.

Original languageEnglish
Article number104281
JournalCellular Immunology
Volume361
DOIs
StatePublished - 1 Mar 2021

Keywords

  • CCR7
  • Calcium signaling
  • IL-1 receptor antagonist
  • Immunomodulation
  • NF-κB

ASJC Scopus subject areas

  • Immunology

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