Differential toxicity response of normal and neoplastic cells in vitro to 3,4-benzopyrene and 3-miethylcholanthrene

L. J. Alfred, A. Globerson, Y. Berwald, R. T. Prehn

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24 Scopus citations

Abstract

Monolayer of normal and neoplastic cells in vitro were exposed to the carcinogenic hydrocarbons, 3,4-benzopyrene (BP) and 3-methylcholanthrene (MC), and to the non-carcinogenic hydrocarbon, chrysene. The normal cells were shown to be highly susceptible to the cytotoxic effects of BP and MC. On the other hand, neoplastic cells produced by these carcinogens, polyoma virus, or spontaneously transformed in vitro were found to be relatively resistant to the cytotoxic effects of the same carcinogens. There was no cytotoxicity of chrysene to normal or neoplastic cells. Measurements of the total cell protein and viable cell numbers of BP-treated cultures derived from normal embryo (C3H) and BP-induced tumour (C7) tissues were made. It was shown that there was an intense toxic response and a suppression of growth of the normal cells exposed to this carcinogen and little if any toxicity or inhibition of growth of the neoplastic cells.

Original languageEnglish
Pages (from-to)159-164
Number of pages6
JournalBritish Journal of Cancer
Volume18
Issue number1
DOIs
StatePublished - 1 Jan 1964
Externally publishedYes

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