Differentiation of human parthenogenetic pluripotent stem cells reveals multiple tissue- and isoform-specific imprinted transcripts

Yonatan Stelzer, Shiran Bar, Osnat Bartok, Shaked Afik, Daniel Ronen, Sebastian Kadener, Nissim Benvenisty

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Parental imprinting results in monoallelic parent-oforigin- dependent gene expression. However, many imprinted genes identified by differential methylation do not exhibit complete monoallelic expression. Previous studies demonstrated complex tissue-dependent expression patterns for some imprinted genes. Still, the complete magnitude of this phenomenon remains largely unknown. By differentiating human parthenogenetic induced pluripotent stem cells into different cell types and combining DNA methylation with a 50 RNA sequencing methodology, we were able to identify tissue- and isoform-dependent imprinted genes in a genome-wide manner.We demonstrate that nearly half of all imprinted genes express both biallelic and monoallelic isoforms that are controlled by tissue-specific alternative promoters. This study provides a global analysis of tissue-specific imprinting in humans and suggests that alternative promoters are central in the regulation of imprinted genes.

Original languageEnglish
Pages (from-to)308-320
Number of pages13
JournalCell Reports
Volume11
Issue number2
DOIs
StatePublished - 1 Jan 2015
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology (all)

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