Diffusion and perfusion magnetic resonance imaging following closed head injury in rats

Yaniv Assaf, Alex Holokovsky, Elisha Berman, Yoram Shapira, Esther Shohami, Yoram Cohen

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58 Scopus citations

Abstract

Diffusion-, perfusion-, T1-, and T2-weighted magnetic resonance imaging (MRI) were performed at 1-2 h, 24 h, and 1 week following closed head injury (CHI) in rats, and data was compared with hematoxylin and eosin histology. At 1-2 h, large areas of low perfusion in the damaged hemisphere overestimate the histological damage. In the first 2 h, the histological damage seems to be a superposition of abnormalities in the T1- and diffusion-weighted images. In areas with more than 10% reduction in the apparent diffusion coefficients (ADCs), reduced regional cerebral blood volume (rCBV) was also observed. The decrease in ADCs and rCBV correlated with r = 0.78. Changes in the MRI parameters revealed the following: (a) Further reduction in ADC occurred from 83 ± 15% at 1-2 h after trauma to 69 ± 9% at 24 h, and 1 week later a marked elevation in the ADC values is observed. (b) Blood perfusion measurements performed 1-2 h posttrauma revealed a pronounced reduction in r-CBV (53 ± 18%) in the damaged hemisphere in all rats. At 24 h postimpact, areas of hyper- and hypoperfusion were observed. One week later, similar perfusion was found in both hemispheres of all rats. (c) T2 hyperintensity at 24 h overestimated the histological damage found at 1 week. At one week following the trauma, the T2 hyperintensity underestimated the histological damage. It is concluded that CHI, which is a heterogeneous insult, should be studied by a combination of MRI techniques. The superposition of the abnormalities seen on T1 and on the diffusion-weighted MR images at early time point represents best the histological damage. Both T2 and rCBV images are less informative in terms of actual histological damage.

Original languageEnglish
Pages (from-to)1165-1176
Number of pages12
JournalJournal of Neurotrauma
Volume16
Issue number12
DOIs
StatePublished - 1 Jan 1999
Externally publishedYes

Keywords

  • Closed head injury
  • Diffusion and perfusion MRI
  • Head trauma
  • MRI
  • Rat brain

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