Direct detection of analyte binding to single molecularly imprinted polymer particles by confocal Raman spectroscopy

Marc Bompart, Levi A. Gheber, Yannick De Wilde, Karsten Haupt

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

We describe the use of Raman spectroscopy to detect and quantify, for the first time, the presence of the imprinting template in single molecularly imprinted polymer microspheres. The polymers were imprinted with the β-blocking drugs propranolol and atenolol, and precipitation polymerization was used to obtain spherical particles of diameters of 200 nm and 1.5 μm. The size of the Raman laser spot being between 1 μm and a few μm, the nanoparticles were used for bulk detection whereas with micrometer-sized particles, quantitative measurements on single particles were possible. The laser power, and consequently the acquisition times, needed to be adapted as a function of the polymer and template used in order to avoid burning. Analyte quantification from Raman spectra is straightforward by determining the peak height of a typical Raman band of the analyte, and by using a typical polymer peak for normalization. Relatively low detection limits down to 1 μM have been reached for the detection of S-propranolol through bulk measurements on MIP nanoparticles.

Original languageEnglish
Pages (from-to)568-571
Number of pages4
JournalBiosensors and Bioelectronics
Volume25
Issue number3
DOIs
StatePublished - 15 Nov 2009

Keywords

  • Confocal Raman spectroscopy
  • Microsphere
  • Molecular imprinting
  • Molecular recognition
  • Synthetic receptor

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

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