Direct effect of CD on glutathione S-transferase and glutathione reductase from Calystegia sepium

Lyudmila Lyubenova, Christine Götz, Avi Golan-Goldhirsh, Peter Schröder

    Research output: Contribution to journalArticlepeer-review

    22 Scopus citations

    Abstract

    Interactions between heavy metals, glutathione, glutathione S-transferase (GST), and glutathione reductase (GR) are being investigated by many working groups, but evaluation of the direct effect of Cd2+ on these enzymes in vitro is lacking. We report here the effect of cadmium (10, 50, 100, 250 μM CdSO4) on partially purified enzymes from Calystegia sepium. Plants were grown under normal field conditions without metals and the enzymes were extracted by Tris buffer and partially purified by ammonium sulphate fractionation and gel filtration. Glutathione S-transferase activity was measured with different substrates, i.e., 1-chloro-2,4-dinitrobenzene (CDNB), p-nitrobenzylchloride (NBC), and the herbicide Fluorodifen. GST activity was significantly lower in leaf compared to stem, flower, and rhizome and the inhibitory effect of Cd was obtained with NBC and Fluorodifen substrates at 250 μM. There was no effect of Cd on GR activity up to 250 μM.

    Original languageEnglish
    Pages (from-to)465-473
    Number of pages9
    JournalInternational Journal of Phytoremediation
    Volume9
    Issue number6
    DOIs
    StatePublished - 1 Nov 2007

    Keywords

    • Calystegia sepium
    • CdSO cadmium
    • Glutathione
    • Glutathione S-transferase
    • Heavy metals

    ASJC Scopus subject areas

    • Environmental Chemistry
    • Pollution
    • Plant Science

    Fingerprint

    Dive into the research topics of 'Direct effect of CD on glutathione S-transferase and glutathione reductase from Calystegia sepium'. Together they form a unique fingerprint.

    Cite this