Dissecting the Roles of the N- and C-Flanking Residues of Acetyllysine Substrates for SIRT1 Activity

Roman Meledin, Ashraf Brik, Amir Aharoni

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

SIRT1 specificity: The multispecific SIRT1 enzyme catalyzes the deacetylation of acetyllysine residues within protein targets. However, little is known regarding the molecular basis for SIRT1 substrate recognition. Kinetic analysis of SIRT1 with a panel of peptide substrates shows the high importance of the region N-flanking the target acetyllysine and its high conservation through evolution.

Original languageEnglish
Pages (from-to)577-581
Number of pages5
JournalChemBioChem
Volume14
Issue number5
DOIs
StatePublished - 1 Mar 2013

Keywords

  • Deacetylation
  • Peptides
  • Sirt1
  • Substrate specificity

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Organic Chemistry

Fingerprint

Dive into the research topics of 'Dissecting the Roles of the N- and C-Flanking Residues of Acetyllysine Substrates for SIRT1 Activity'. Together they form a unique fingerprint.

Cite this