Dissecting the Roles of the N- and C-Flanking Residues of Acetyllysine Substrates for SIRT1 Activity

Roman Meledin; Ashraf Brik; Amir Aharoni

doi:10.1002/cbic.201200727

Dissecting the Roles of the N- and C-Flanking Residues of Acetyllysine Substrates for SIRT1 Activity

Roman Meledin, Ashraf Brik, Amir Aharoni

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

SIRT1 specificity: The multispecific SIRT1 enzyme catalyzes the deacetylation of acetyllysine residues within protein targets. However, little is known regarding the molecular basis for SIRT1 substrate recognition. Kinetic analysis of SIRT1 with a panel of peptide substrates shows the high importance of the region N-flanking the target acetyllysine and its high conservation through evolution.

Original language	English
Pages (from-to)	577-581
Number of pages	5
Journal	ChemBioChem
Volume	14
Issue number	5
DOIs	https://doi.org/10.1002/cbic.201200727
State	Published - 1 Mar 2013

Keywords

Deacetylation
Peptides
Sirt1
Substrate specificity

ASJC Scopus subject areas

Biochemistry
Molecular Medicine
Molecular Biology
Organic Chemistry

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10.1002/cbic.201200727

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abstract = "SIRT1 specificity: The multispecific SIRT1 enzyme catalyzes the deacetylation of acetyllysine residues within protein targets. However, little is known regarding the molecular basis for SIRT1 substrate recognition. Kinetic analysis of SIRT1 with a panel of peptide substrates shows the high importance of the region N-flanking the target acetyllysine and its high conservation through evolution.",

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AU - Aharoni, Amir

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AB - SIRT1 specificity: The multispecific SIRT1 enzyme catalyzes the deacetylation of acetyllysine residues within protein targets. However, little is known regarding the molecular basis for SIRT1 substrate recognition. Kinetic analysis of SIRT1 with a panel of peptide substrates shows the high importance of the region N-flanking the target acetyllysine and its high conservation through evolution.

KW - Deacetylation

KW - Peptides

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KW - Substrate specificity

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Dissecting the Roles of the N- and C-Flanking Residues of Acetyllysine Substrates for SIRT1 Activity

Abstract

Keywords

ASJC Scopus subject areas

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