Distinct axonemal processes underlie spontaneous and stimulated airway ciliary activity

Weiyuan Ma, Shai D. Silberberg, Zvi Priel

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

Cilia are small organelles protruding from the cell surface that beat synchronously, producing biological transport. Despite intense research for over a century, the mechanisms underlying ciliary beating are still not well understood. Even the nature of the cytosolic molecules required for spontaneous and stimulated beating is debatable. In an effort to resolve fundamental questions related to cilia beating, we developed a method that integrates the whole-cell mode of the patch-clamp technique with ciliary beat frequency measurements on a single cell. This method enables to control the composition of the intracellular solution while the cilia remain intact, thus providing a unique tool to simultaneously investigate the biochemical and physiological mechanism of ciliary beating. Thus far, we investigated whether the spontaneous and stimulated states of cilia beating are controlled by the same intracellular molecular mechanisms. It was found that: (a) MgATP was sufficient to support spontaneous beating. (b) Ca2+ alone or Ca2+-calmodulin at concentrations as high as 1 μM could not alter ciliary beating. (c) In the absence of Ca2+, cyclic nucleotides produced a moderate rise in ciliary beating while in the presence of Ca2+ robust enhancement was observed. These results suggest that the axonemal machinery can function in at least two different modes.

Original languageEnglish
Pages (from-to)875-885
Number of pages11
JournalJournal of General Physiology
Volume120
Issue number6
DOIs
StatePublished - 1 Dec 2002

Keywords

  • Calcium signaling
  • Cilia
  • Cyclic nucleotides
  • Molecular motors
  • Patch-clamp techniques

Fingerprint

Dive into the research topics of 'Distinct axonemal processes underlie spontaneous and stimulated airway ciliary activity'. Together they form a unique fingerprint.

Cite this