Diverse subcellular locations of cryptogein-induced reactive oxygen species production in tobacco bright yellow-2 cells

Cher Ashtamker, Vladimir Kiss, Moshe Sagi, Olga Davydov, Robert Fluhr

    Research output: Contribution to journalArticlepeer-review

    120 Scopus citations

    Abstract

    Reactive oxygen species (ROS) play a crucial role in many cellular responses and signaling pathways, including the oxidative burst defense response to pathogens. We have examined very early events in cryptogein-induced ROS production in tobacco (Nicotiana tabacum) Bright Yellow-2 suspension cells. Using Amplex Red and Amplex Ultra Red reagents, which report real-time H 2O2 accumulation in cell populations, we show that the internal signal for H2O2 develops more rapidly than the external apoplastic signal. Subcellular accumulation of H2O 2 was also followed in individual cells using the 2′,7′-dichlorofluorescein diacetate fluorescent probe. Major accumulation was detected in endomembrane, cytoplasmic, and nuclear compartments. When cryptogein was added, the signal developed first in the nuclear region and, after a short delay, in the cell periphery. Interestingly, isolated nuclei were capable of producing H2O2 in a calcium-dependent manner, implying that nuclei can serve as a potential active source of ROS production. These results show complex spatial compartmentalization for ROS accumulation and an unexpected temporal sequence of events that occurs after cryptogein application, suggesting novel intricacy in ROS-signaling cascades.

    Original languageEnglish
    Pages (from-to)1817-1826
    Number of pages10
    JournalPlant Physiology
    Volume143
    Issue number4
    DOIs
    StatePublished - 1 Jan 2007

    ASJC Scopus subject areas

    • Physiology
    • Genetics
    • Plant Science

    Fingerprint

    Dive into the research topics of 'Diverse subcellular locations of cryptogein-induced reactive oxygen species production in tobacco bright yellow-2 cells'. Together they form a unique fingerprint.

    Cite this