TY - JOUR
T1 - Diversity of bacterial biota in Capnodis tenebrionis (Coleoptera
T2 - Buprestidae) larvae
AU - Barak, Hana
AU - Kumar, Pradeep
AU - Zaritsky, Arieh
AU - Mendel, Zvi
AU - Ment, Dana
AU - Kushmaro, Ariel
AU - Ben-Dov, Eitan
N1 - Funding Information:
This study was supported by Grant # 857-0684-13 of the Chief Scientist, Israel Ministry of Agriculture (to A.K., Z.M., E.B.-D., and A.Z.). Scholarship (for P.K.) from the Council for Higher Education (VATAT) of Israel.
Publisher Copyright:
© 2019 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2019/3/1
Y1 - 2019/3/1
N2 - The bacterial biota in larvae of Capnodis tenebrionis, a serious pest of cultivated stone-fruit trees in the West Palearctic, was revealed for the first time using the MiSeq platform. The core bacterial community remained the same in neonates whether upon hatching or grown on peach plants or an artificial diet, suggesting that C. tenebrionis larvae acquire much of their bacterial biome from the parent adult. Reads affiliated with class levels Gammaproteobacteria and Alphaproteobacteria (phylum Proteobacteria ca. 86%), and Actinobacteria (ca. 14%) were highly abundant. Most diverse reads belong to the families Xanthomonadaceae (50%), Methylobacteriaceae (20%), Hyphomicrobiaceae (9%), Micrococcaceae (7%) and Geodermatophilaceae (4.5%). About two-thirds of the reads are affiliated with the genera Lysobacter, Microvirga, Methylobacterium, and Arthrobacter, which encompass species displaying cellulolytic and lipolytic activities. This study provides a foundation for future studies to elucidate the roles of bacterial biota in C. tenebrionis.
AB - The bacterial biota in larvae of Capnodis tenebrionis, a serious pest of cultivated stone-fruit trees in the West Palearctic, was revealed for the first time using the MiSeq platform. The core bacterial community remained the same in neonates whether upon hatching or grown on peach plants or an artificial diet, suggesting that C. tenebrionis larvae acquire much of their bacterial biome from the parent adult. Reads affiliated with class levels Gammaproteobacteria and Alphaproteobacteria (phylum Proteobacteria ca. 86%), and Actinobacteria (ca. 14%) were highly abundant. Most diverse reads belong to the families Xanthomonadaceae (50%), Methylobacteriaceae (20%), Hyphomicrobiaceae (9%), Micrococcaceae (7%) and Geodermatophilaceae (4.5%). About two-thirds of the reads are affiliated with the genera Lysobacter, Microvirga, Methylobacterium, and Arthrobacter, which encompass species displaying cellulolytic and lipolytic activities. This study provides a foundation for future studies to elucidate the roles of bacterial biota in C. tenebrionis.
KW - Bacterial biota
KW - Buprestidae
KW - Capnodis
KW - Stonefruit
UR - http://www.scopus.com/inward/record.url?scp=85060183436&partnerID=8YFLogxK
U2 - 10.3390/pathogens8010004
DO - 10.3390/pathogens8010004
M3 - Article
AN - SCOPUS:85060183436
VL - 8
JO - Pathogens
JF - Pathogens
SN - 2076-0817
IS - 1
M1 - 4
ER -
