DNA-CNT nanowire networks for DNA detection

Yossi Weizmann, David M. Chenoweth, Timothy M. Swager

Research output: Contribution to journalArticlepeer-review

71 Scopus citations

Abstract

The ability to detect biological analytes in a rapid, sensitive, operationally simple, and cost-effective manner will impact human health and safety. Hybrid biocatalyzed-carbon nanotube (CNT) nanowire-based detection methods offer a highly sensitive and specific platform for the fabrication of simple and effective conductometric devices. Here, we report a conductivity-based DNA detection method utilizing carbon nanotube-DNA nanowire devices and oligonucleotide-functionalized enzyme probes. Key to our sensor design is a DNA-linked-CNT wire motif, which forms a network of interrupted carbon nanotube wires connecting two electrodes. Sensing occurs at the DNA junctions linking CNTs, followed by amplification using enzymatic metalization leading to a conductimetric response. The DNA analyte detection limit is 10 fM with the ability to discriminate single, double, and triple base pair mismatches. DNA-CNT nanowires and device sensing gaps were characterized by scanning electron microscopy (SEM) and confocal Raman microscopy, supporting the enhanced conductometric response resulting from nanowire metallization.

Original languageEnglish
Pages (from-to)3238-3241
Number of pages4
JournalJournal of the American Chemical Society
Volume133
Issue number10
DOIs
StatePublished - 16 Mar 2011
Externally publishedYes

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