DNA markers for sex: Molecular evidence for gender dimorphism in dioecious Mercurialis annua L.

D. K. Khadka; A. Nejidat; M. Tal; A. Golan-Goldhirsh

doi:10.1023/A:1020361424758

DNA markers for sex: Molecular evidence for gender dimorphism in dioecious Mercurialis annua L.

D. K. Khadka, A. Nejidat, M. Tal, A. Golan-Goldhirsh

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

34 Scopus citations

Abstract

Male specific Random Amplified Polymorphic DNA (RAPD) markers, OPB01-1562 and OPC07-303, were identified and sequenced in dioecious Mercurialis annua. Sequence Characterized Amplified Region (SCAR) primers were designed. Several internal segments of OPB01-1562 were amplified as male specific SCAR markers. These markers were PCR amplified from strong, intermediate and weak male subtypes selected according to their resistance to feminization by cytokinin. Nucleotide sequence of OPB01-1562 isolated from three male subtypes were near identical. The OPB01-1562 and derived SCAR markers were absent in females as well as hexaploid Mercurialis male and monoecious individuals. The gender relationship of the markers was maintained in all ecotypes tested. There were 2 internal fragments of OPB01-1562, which were PCR amplified from all genotypes of diploid and hexaploid Mercurialis. It is argued that identification of gender specific DNA suggests a dimorphic differentiation of the genome of dioecious Mercurialis annua.

Original language	English
Pages (from-to)	251-257
Number of pages	7
Journal	Molecular Breeding
Volume	9
Issue number	4
DOIs	https://doi.org/10.1023/A:1020361424758
State	Published - 16 Oct 2002

Keywords

Dioecious
Gender
Mercurialis annua
RAPD
SCAR

ASJC Scopus subject areas

Biotechnology
Molecular Biology
Agronomy and Crop Science
Genetics
Plant Science

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title = "DNA markers for sex: Molecular evidence for gender dimorphism in dioecious Mercurialis annua L.",

abstract = "Male specific Random Amplified Polymorphic DNA (RAPD) markers, OPB01-1562 and OPC07-303, were identified and sequenced in dioecious Mercurialis annua. Sequence Characterized Amplified Region (SCAR) primers were designed. Several internal segments of OPB01-1562 were amplified as male specific SCAR markers. These markers were PCR amplified from strong, intermediate and weak male subtypes selected according to their resistance to feminization by cytokinin. Nucleotide sequence of OPB01-1562 isolated from three male subtypes were near identical. The OPB01-1562 and derived SCAR markers were absent in females as well as hexaploid Mercurialis male and monoecious individuals. The gender relationship of the markers was maintained in all ecotypes tested. There were 2 internal fragments of OPB01-1562, which were PCR amplified from all genotypes of diploid and hexaploid Mercurialis. It is argued that identification of gender specific DNA suggests a dimorphic differentiation of the genome of dioecious Mercurialis annua.",

keywords = "Dioecious, Gender, Mercurialis annua, RAPD, SCAR",

author = "Khadka, {D. K.} and A. Nejidat and M. Tal and A. Golan-Goldhirsh",

note = "Funding Information: We thank Dr Oz Barazani for his assistance in preparation of samples for sequencing in the final stages of the project. This work was partly supported by the Rashi Foundation and the AID Grant No. TA-MOU-98-CA17-028.",

year = "2002",

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doi = "10.1023/A:1020361424758",

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AU - Tal, M.

AU - Golan-Goldhirsh, A.

N1 - Funding Information: We thank Dr Oz Barazani for his assistance in preparation of samples for sequencing in the final stages of the project. This work was partly supported by the Rashi Foundation and the AID Grant No. TA-MOU-98-CA17-028.

PY - 2002/10/16

Y1 - 2002/10/16

N2 - Male specific Random Amplified Polymorphic DNA (RAPD) markers, OPB01-1562 and OPC07-303, were identified and sequenced in dioecious Mercurialis annua. Sequence Characterized Amplified Region (SCAR) primers were designed. Several internal segments of OPB01-1562 were amplified as male specific SCAR markers. These markers were PCR amplified from strong, intermediate and weak male subtypes selected according to their resistance to feminization by cytokinin. Nucleotide sequence of OPB01-1562 isolated from three male subtypes were near identical. The OPB01-1562 and derived SCAR markers were absent in females as well as hexaploid Mercurialis male and monoecious individuals. The gender relationship of the markers was maintained in all ecotypes tested. There were 2 internal fragments of OPB01-1562, which were PCR amplified from all genotypes of diploid and hexaploid Mercurialis. It is argued that identification of gender specific DNA suggests a dimorphic differentiation of the genome of dioecious Mercurialis annua.

AB - Male specific Random Amplified Polymorphic DNA (RAPD) markers, OPB01-1562 and OPC07-303, were identified and sequenced in dioecious Mercurialis annua. Sequence Characterized Amplified Region (SCAR) primers were designed. Several internal segments of OPB01-1562 were amplified as male specific SCAR markers. These markers were PCR amplified from strong, intermediate and weak male subtypes selected according to their resistance to feminization by cytokinin. Nucleotide sequence of OPB01-1562 isolated from three male subtypes were near identical. The OPB01-1562 and derived SCAR markers were absent in females as well as hexaploid Mercurialis male and monoecious individuals. The gender relationship of the markers was maintained in all ecotypes tested. There were 2 internal fragments of OPB01-1562, which were PCR amplified from all genotypes of diploid and hexaploid Mercurialis. It is argued that identification of gender specific DNA suggests a dimorphic differentiation of the genome of dioecious Mercurialis annua.

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DNA markers for sex: Molecular evidence for gender dimorphism in dioecious Mercurialis annua L.

Abstract

Keywords

ASJC Scopus subject areas

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