DNA origami nanorobot fiber optic genosensor to TMV

Emanuela Torelli, Marisa Manzano, Sachin K. Srivastava, Robert S. Marks

Research output: Contribution to journalArticlepeer-review

17 Scopus citations


In the quest of greater sensitivity and specificity of diagnostic systems, one continually searches for alternative DNA hybridization methods, enabling greater versatility and where possible field-enabled detection of target analytes. We present, herein, a hybrid molecular self-assembled scaffolded DNA origami entity, intimately immobilized via capture probes linked to aminopropyltriethoxysilane, onto a glass optical fiber end-face transducer, thus producing a novel biosensor. Immobilized DNA nanorobots with a switchable flap can then be actuated by a specific target DNA present in a sample, by exposing a hemin/G-quadruplex DNAzyme, which then catalyzes the generation of chemiluminescence, once the specific fiber probes are immersed in a luminol-based solution. Integrating organic nanorobots to inorganic fiber optics creates a hybrid system that we demonstrate as a proof-of-principle can be utilized in specific DNA sequence detection. This system has potential applications in a wide range of fields, including point-of-care diagnostics or cellular in vivo biosensing when using ultrathin fiber optic probes for research purposes.

Original languageEnglish
Pages (from-to)209-215
Number of pages7
JournalBiosensors and Bioelectronics
StatePublished - 15 Jan 2018


  • 3D DNA origami nanorobot
  • Genosensor
  • Optical fiber
  • Switchable nanodevice

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry


Dive into the research topics of 'DNA origami nanorobot fiber optic genosensor to TMV'. Together they form a unique fingerprint.

Cite this