DNA Sequence Context Controls the Binding and Processivity of the T7 DNA Primase

Ariel Afek, Stefan Ilic, John Horton, David B. Lukatsky, Raluca Gordan, Barak Akabayov

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Primases are key enzymes involved in DNA replication. They act on single-stranded DNA and catalyze the synthesis of short RNA primers used by DNA polymerases. Here, we investigate the DNA binding and activity of the bacteriophage T7 primase using a new workflow called high-throughput primase profiling (HTPP). Using a unique combination of high-throughput binding assays and biochemical analyses, HTPP reveals a complex landscape of binding specificity and functional activity for the T7 primase, determined by sequences flanking the primase recognition site. We identified specific features, such as G/T-rich flanks, which increase primase-DNA binding up to 10-fold and, surprisingly, also increase the length of newly formed RNA (up to 3-fold). To our knowledge, variability in primer length has not been reported for this primase. We expect that applying HTPP to additional enzymes will reveal new insights into the effects of DNA sequence composition on the DNA recognition and functional activity of primases.

Original languageEnglish
Pages (from-to)141-147
Number of pages7
JournaliScience
Volume2
DOIs
StatePublished - 27 Apr 2018

Keywords

  • Biochemical Mechanism
  • Molecular Biology
  • Molecular Genetics

ASJC Scopus subject areas

  • General

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