TY - JOUR
T1 - Dosage differences in 12-OXOPHYTODIENOATE REDUCTASE genes modulate wheat root growth
AU - Gabay, Gilad
AU - Wang, Hanchao
AU - Zhang, Junli
AU - Moriconi, Jorge I.
AU - Burguener, German F.
AU - Gualano, Leonardo D.
AU - Howell, Tyson
AU - Lukaszewski, Adam
AU - Staskawicz, Brian
AU - Cho, Myeong Je
AU - Tanaka, Jaclyn
AU - Fahima, Tzion
AU - Ke, Haiyan
AU - Dehesh, Katayoon
AU - Zhang, Guo Liang
AU - Gou, Jin Ying
AU - Hamberg, Mats
AU - Santa-María, Guillermo E.
AU - Dubcovsky, Jorge
N1 - Publisher Copyright:
© 2023, The Author(s).
PY - 2023/12/1
Y1 - 2023/12/1
N2 - Wheat, an essential crop for global food security, is well adapted to a wide variety of soils. However, the gene networks shaping different root architectures remain poorly understood. We report here that dosage differences in a cluster of monocot-specific 12-OXOPHYTODIENOATE REDUCTASE genes from subfamily III (OPRIII) modulate key differences in wheat root architecture, which are associated with grain yield under water-limited conditions. Wheat plants with loss-of-function mutations in OPRIII show longer seminal roots, whereas increased OPRIII dosage or transgenic over-expression result in reduced seminal root growth, precocious development of lateral roots and increased jasmonic acid (JA and JA-Ile). Pharmacological inhibition of JA-biosynthesis abolishes root length differences, consistent with a JA-mediated mechanism. Transcriptome analyses of transgenic and wild-type lines show significant enriched JA-biosynthetic and reactive oxygen species (ROS) pathways, which parallel changes in ROS distribution. OPRIII genes provide a useful entry point to engineer root architecture in wheat and other cereals.
AB - Wheat, an essential crop for global food security, is well adapted to a wide variety of soils. However, the gene networks shaping different root architectures remain poorly understood. We report here that dosage differences in a cluster of monocot-specific 12-OXOPHYTODIENOATE REDUCTASE genes from subfamily III (OPRIII) modulate key differences in wheat root architecture, which are associated with grain yield under water-limited conditions. Wheat plants with loss-of-function mutations in OPRIII show longer seminal roots, whereas increased OPRIII dosage or transgenic over-expression result in reduced seminal root growth, precocious development of lateral roots and increased jasmonic acid (JA and JA-Ile). Pharmacological inhibition of JA-biosynthesis abolishes root length differences, consistent with a JA-mediated mechanism. Transcriptome analyses of transgenic and wild-type lines show significant enriched JA-biosynthetic and reactive oxygen species (ROS) pathways, which parallel changes in ROS distribution. OPRIII genes provide a useful entry point to engineer root architecture in wheat and other cereals.
UR - https://www.scopus.com/pages/publications/85147234972
U2 - 10.1038/s41467-023-36248-y
DO - 10.1038/s41467-023-36248-y
M3 - Article
C2 - 36725858
AN - SCOPUS:85147234972
SN - 2041-1723
VL - 14
JO - Nature Communications
JF - Nature Communications
IS - 1
M1 - 539
ER -
