Droplet-based microfluidic platforms for single T cell secretion analysis of IL-10 cytokine

Tania Konry; Margarita Dominguez-Villar; Clare Baecher-Allan; David A. Hafler; Martin L. Yarmush

doi:10.1016/j.bios.2010.09.006

Droplet-based microfluidic platforms for single T cell secretion analysis of IL-10 cytokine

Tania Konry
, Margarita Dominguez-Villar
, Clare Baecher-Allan
, David A. Hafler
, Martin L. Yarmush

Research output: Contribution to journal › Article › peer-review

119 Scopus citations

Abstract

Here we present a microfluidic method for the analysis of single cell secretions. The method co-encapsulates cells with microspheres conjugated with capture antibodies and detection fluorescence-labeled antibodies. The secreted substance captured on the microsphere surface and detected via detection antibodies generating a localized fluorescent signal on a microsphere surface. Using this method, CD4+CD25+ regulatory T cells were encapsulated and assayed to detect IL-10 secreting cell in population.

Original language	English
Pages (from-to)	2707-2710
Number of pages	4
Journal	Biosensors and Bioelectronics
Volume	26
Issue number	5
DOIs	https://doi.org/10.1016/j.bios.2010.09.006
State	Published - 15 Jan 2011
Externally published	Yes

Keywords

Cell secretion
Droplets
Microfluidic
Microsphere
Single cell

ASJC Scopus subject areas

Biotechnology
Biophysics
Biomedical Engineering
Electrochemistry

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10.1016/j.bios.2010.09.006

Cite this

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title = "Droplet-based microfluidic platforms for single T cell secretion analysis of IL-10 cytokine",

abstract = "Here we present a microfluidic method for the analysis of single cell secretions. The method co-encapsulates cells with microspheres conjugated with capture antibodies and detection fluorescence-labeled antibodies. The secreted substance captured on the microsphere surface and detected via detection antibodies generating a localized fluorescent signal on a microsphere surface. Using this method, CD4+CD25+ regulatory T cells were encapsulated and assayed to detect IL-10 secreting cell in population.",

keywords = "Cell secretion, Droplets, Microfluidic, Microsphere, Single cell",

author = "Tania Konry and Margarita Dominguez-Villar and Clare Baecher-Allan and Hafler, \{David A.\} and Yarmush, \{Martin L.\}",

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T1 - Droplet-based microfluidic platforms for single T cell secretion analysis of IL-10 cytokine

AU - Konry, Tania

AU - Dominguez-Villar, Margarita

AU - Baecher-Allan, Clare

AU - Hafler, David A.

AU - Yarmush, Martin L.

PY - 2011/1/15

Y1 - 2011/1/15

N2 - Here we present a microfluidic method for the analysis of single cell secretions. The method co-encapsulates cells with microspheres conjugated with capture antibodies and detection fluorescence-labeled antibodies. The secreted substance captured on the microsphere surface and detected via detection antibodies generating a localized fluorescent signal on a microsphere surface. Using this method, CD4+CD25+ regulatory T cells were encapsulated and assayed to detect IL-10 secreting cell in population.

AB - Here we present a microfluidic method for the analysis of single cell secretions. The method co-encapsulates cells with microspheres conjugated with capture antibodies and detection fluorescence-labeled antibodies. The secreted substance captured on the microsphere surface and detected via detection antibodies generating a localized fluorescent signal on a microsphere surface. Using this method, CD4+CD25+ regulatory T cells were encapsulated and assayed to detect IL-10 secreting cell in population.

KW - Cell secretion

KW - Droplets

KW - Microfluidic

KW - Microsphere

KW - Single cell

UR - https://www.scopus.com/pages/publications/78650611056

U2 - 10.1016/j.bios.2010.09.006

DO - 10.1016/j.bios.2010.09.006

M3 - Article

C2 - 20888750

AN - SCOPUS:78650611056

SN - 0956-5663

VL - 26

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EP - 2710

JO - Biosensors and Bioelectronics

JF - Biosensors and Bioelectronics

IS - 5

ER -

Droplet-based microfluidic platforms for single T cell secretion analysis of IL-10 cytokine

Abstract

Keywords

ASJC Scopus subject areas

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