Abstract
Current techniques of biomarker detection employ population-based studies, which fail to provide information on cellular and molecular heterogeneity and variances in functional behavior, which is important for diseased state characterization. Additionally, low concentration of samples in biological fluids creates hurdles in detection using these methods. Thus, improvement in detection techniques of clinical biomarkers has become imperative to halt disease progression at the earliest possible stage. Droplet microfluidics has become quite popular as a diagnostic platform due to its ability to sensitively detect low volume of clinical samples in a rapid and high-throughput manner. These advantages make the droplets an ideal platform for low-abundance biomarker detection. This chapter focuses on various techniques for detection of surface and secreted protein biomarkers using droplet microfluidics platform.
| Original language | English |
|---|---|
| Title of host publication | Microfluidic Methods for Molecular Biology |
| Publisher | Springer International Publishing |
| Pages | 219-233 |
| Number of pages | 15 |
| ISBN (Electronic) | 9783319300191 |
| ISBN (Print) | 9783319300177 |
| DOIs | |
| State | Published - 1 Jan 2016 |
| Externally published | Yes |
Keywords
- Diagnostics
- Droplet microfluidics
- Secretory proteins
- Single cell analysis
- Surfacemarker proteins
ASJC Scopus subject areas
- General Medicine
- General Immunology and Microbiology
- General Engineering
- General Chemical Engineering