TY - GEN
T1 - Dynamic Laser Speckle Imaging for Fast Evaluation of the Antibacterial Susceptibility by the Disc Diffusion Method
AU - Lihacova, Ilze
AU - Balmages, Ilya
AU - Reinis, Aigars
AU - Kistkins, Svjatoslavs
AU - Bliznuks, Dmitrijs
AU - Plorina, Emilija Vija
AU - Lihachev, Alexey
N1 - Publisher Copyright:
© The Author(s), under exclusive license to Springer Nature Switzerland AG 2023.
PY - 2023/1/1
Y1 - 2023/1/1
N2 - Phenotypic resistance tests e.g., disc diffusion method require 16–24 h to obtain the results, while the PCR tests provide only genotypic type of antibacterial resistance. New methods are needed to assess antibacterial resistance faster than existing methods, thus providing targeted pharmacological intervention at the early stage of the disease, increasing a patient’s survival chances. Laser speckle imaging technique allows tracking moving particles in optically inhomogeneous media, allowing to observe the processes of changing the behavior of the sterile zone. The choice and adaptation of a mathematical model describing changes in the growth diameter of the sterile zone as a function of time and comparison with experimental data will help to understand and describe the formation of the sterile zone. The aim is to create processing algorithms for the obtained images to predict the diameter at the end of the disc diffusion test from the diameter of the earliest visible sterile zone.
AB - Phenotypic resistance tests e.g., disc diffusion method require 16–24 h to obtain the results, while the PCR tests provide only genotypic type of antibacterial resistance. New methods are needed to assess antibacterial resistance faster than existing methods, thus providing targeted pharmacological intervention at the early stage of the disease, increasing a patient’s survival chances. Laser speckle imaging technique allows tracking moving particles in optically inhomogeneous media, allowing to observe the processes of changing the behavior of the sterile zone. The choice and adaptation of a mathematical model describing changes in the growth diameter of the sterile zone as a function of time and comparison with experimental data will help to understand and describe the formation of the sterile zone. The aim is to create processing algorithms for the obtained images to predict the diameter at the end of the disc diffusion test from the diameter of the earliest visible sterile zone.
KW - antibacterial resistance estimation
KW - disc diffusion method
KW - image processing
KW - laser speckle imaging
KW - phenotypic antibacterial resistance
KW - sub-pixel correlation analysis
UR - http://www.scopus.com/inward/record.url?scp=85175853043&partnerID=8YFLogxK
U2 - 10.1007/978-3-031-37132-5_39
DO - 10.1007/978-3-031-37132-5_39
M3 - Conference contribution
AN - SCOPUS:85175853043
SN - 9783031371318
T3 - IFMBE Proceedings
SP - 313
EP - 319
BT - 19th Nordic-Baltic Conference on Biomedical Engineering and Medical Physics - Proceedings of NBC 2023
A2 - Dekhtyar, Yuri
A2 - Saknite, Inga
PB - Springer Science and Business Media Deutschland GmbH
T2 - 19th Nordic-Baltic Conference on Biomedical Engineering and Medical Physics, NBC 2023
Y2 - 12 June 2023 through 14 June 2023
ER -