Early detection of premalignant changes in cell cultures using light-induced fluorescence spectroscopy

E. Bogomolny, Shaul Mordechai, A. Zwielly, M. Huleihel

Research output: Contribution to journalArticlepeer-review

14 Scopus citations


Light-induced fluorescence (LIF) spectroscopy has demonstrated ability as a novel, noninvasive and sensitive technology for early detection of cancer. The goal of the present study is to examine the potential of this spectroscopic method for early detection and characterization of premalignant changes. As a model we used both cell lines and primary cells, which were transformed to malignant by retrovirus. Fluorescence measurements and morphological observations of the infected cells were performed at various postinfection times. Our results showed gradual attenuation of fluorescence intensities due to cancer progression which corresponds to aromatic amino acids and nicotinamide adenine dinucleotide (NADH) molecules. In order to obtain grading and supervised classifications of the spectral premalignant changes we used approaches of linear discriminant analysis. The classifications based on Mahalanobis distances allowed us to demonstrate that the accuracy of identification of premalignant stages varied between 83.1% and 96.4%. In summary, we conclude that LIF in tandem with proper statistical tools may be a promising technique for early detection of malignant progression.

Original languageEnglish
Pages (from-to)971-980
Number of pages10
JournalEuropean Biophysics Journal
Issue number7
StatePublished - 1 Sep 2009


  • Discriminant classification function
  • Early diagnosis
  • Light-induced fluorescence
  • Linear discriminant analysis
  • Malignant transformation

ASJC Scopus subject areas

  • Biophysics


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