Effect of acidic pH on heat shock gene expression in Leishmania

Srinivas Garlapati, Edit Dahan, Michal Shapira

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

Temperature and pH shifts trigger differential gene expression and stage transformation in Leishmania. The parasites encounter dramatic fluctuations in the extra-cellular pH between the mid-gut of the sand fly (pH>8) and the phagolysosomal vacuole of mammalian macrophages (pH<6). The authors examined the effect of pH shifts on heat shock gene expression in Leishmania amazonensis and Leishmania donovani promastigotes. Acidic pH resulted in preferential stability of the hsp83 transcripts at 26°C, but hsp transcripts were not preferentially translated as observed during heat shock. Pre-conditioning of promastigotes to acidic pH did not alter the temperature threshold for hsp synthesis but lead to an increase in hsp synthesis mainly in L. donovani at 37°C, and to a slight decrease in the arrest of tubulin synthesis in L. amazonensis. The stage specific morphological alterations that take place in vitro correlated with the arrest in tubulin synthesis and occurred at different temperatures in L. donovani and L. amazonensis. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)95-101
Number of pages7
JournalMolecular and Biochemical Parasitology
Volume100
Issue number1
DOIs
StatePublished - 15 Apr 1999

Keywords

  • Differential gene expression
  • Leishmania amazonensis
  • Leishmania donovani
  • pH shock

ASJC Scopus subject areas

  • Parasitology
  • Molecular Biology

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