Abstract
It is a major interest in the field of hematopoietic stem cell transplantation to reduce scarring of healing wounds with overdeposition of collagen due to radiation injury or graft-versus-host disease. Halofuginone (HF) inhibits collagen α1(I) gene expression and overdeposition of collagen. We examined the effect of HF on the healing of full-depth incision wounds inflicted in normal skin or skin areas compromised by local preirradiation with 18 Gy. Preirradiation significantly decreased the tensile strength of the healing wounds at day 14 (by ∼60%, p < 0.0001). In contrast, HF treatment did not significantly decrease the strength of wounds inflicted in both normal and preirradiated skin. Histological evaluation revealed that HF induced moderate thinning of the dermis accompanied by elevated thickness of the epidermis and enhanced rejoining of subdermal muscles in the wound area. HF only minimally reduced total collagen deposition in both groups, with minor changes in the level of more matured fibrillar collagen network. Our study demonstrates that HF does not significantly affect wound strength. This encourages the possible use of HF as an antifibrotic agent with minimal complications for post-hematopoietic stem cell transplantation complications including radiation toxicity and graft-versus-host disease.
Original language | English |
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Pages (from-to) | 77-83 |
Number of pages | 7 |
Journal | Acta Haematologica |
Volume | 118 |
Issue number | 2 |
DOIs | |
State | Published - 1 Sep 2007 |
Externally published | Yes |
Keywords
- Collagen
- Graft-versus-host disease
- Halofuginone
- Hematopoietic stem cells transplantation
- Ionizing radiation
- Wound healing
ASJC Scopus subject areas
- Hematology