TY - JOUR
T1 - Effect of laser welding with human serum albumin on the expression of P-selectin on platelets
AU - Kirman, I
AU - Lauto, A
AU - Phillips, A
AU - Hamawy, A
AU - Heldman, E
AU - Cuomo, B
AU - Shin, S J
AU - Soslow, R
AU - Felsen, D
AU - Poppas, D P
N1 - Copyright 1999 Wiley-Liss, Inc.
PY - 1999/12/15
Y1 - 1999/12/15
N2 - BACKGROUND AND OBJECTIVE: Artery repair by means of laser energy induces activation of platelets with a risk of thrombosis and local inflammatory reactions. The aim of this study was to investigate the effect of human serum albumin, the most common solder in laser surgery, on platelet activation.STUDY DESIGN/MATERIALS AND METHODS: Platelet activation was evaluated in canine blood by using two-color flow cytometry with a phycoerythrin-labeled antibody to a common platelet marker, glycoprotein IIb/IIIa and fluorescein isothiocyanate-labeled antibody to a platelet activation molecule, P-selectin. Human serum albumin was applied in vitro and in vivo, as a solder during laser reconstruction of canine arteries.RESULTS: In vitro, albumin significantly (P < 0.01) reduces the expression of P-selectin on platelets. This is most likely related to the blockage of P-selectin by albumin, which binds to the platelet surface, as confirmed by flow cytometry with fluorescein isothiocyanate-labeled albumin. In vivo, application of albumin solder tended to result in a lower percentage of P-selectin-expressing platelets in laser-repaired arteries compared to suture-repaired arteries.CONCLUSION: Albumin decreases the percentage of P-selectin-expressing platelets in vitro. Further research may allow the platelet activation inhibiting properties of albumin to be further optimized in vivo.
AB - BACKGROUND AND OBJECTIVE: Artery repair by means of laser energy induces activation of platelets with a risk of thrombosis and local inflammatory reactions. The aim of this study was to investigate the effect of human serum albumin, the most common solder in laser surgery, on platelet activation.STUDY DESIGN/MATERIALS AND METHODS: Platelet activation was evaluated in canine blood by using two-color flow cytometry with a phycoerythrin-labeled antibody to a common platelet marker, glycoprotein IIb/IIIa and fluorescein isothiocyanate-labeled antibody to a platelet activation molecule, P-selectin. Human serum albumin was applied in vitro and in vivo, as a solder during laser reconstruction of canine arteries.RESULTS: In vitro, albumin significantly (P < 0.01) reduces the expression of P-selectin on platelets. This is most likely related to the blockage of P-selectin by albumin, which binds to the platelet surface, as confirmed by flow cytometry with fluorescein isothiocyanate-labeled albumin. In vivo, application of albumin solder tended to result in a lower percentage of P-selectin-expressing platelets in laser-repaired arteries compared to suture-repaired arteries.CONCLUSION: Albumin decreases the percentage of P-selectin-expressing platelets in vitro. Further research may allow the platelet activation inhibiting properties of albumin to be further optimized in vivo.
KW - Animals
KW - Blood Platelets/metabolism
KW - Carotid Arteries/surgery
KW - Dogs
KW - Female
KW - Flow Cytometry
KW - In Vitro Techniques
KW - Laser Therapy/methods
KW - P-Selectin/blood
KW - Platelet Activation/drug effects
KW - Platelet Membrane Glycoproteins/drug effects
KW - Serum Albumin/pharmacology
KW - Tissue Adhesives/pharmacology
UR - http://www.scopus.com/inward/record.url?scp=0033451750&partnerID=8YFLogxK
U2 - 10.1002/(sici)1096-9101(1999)25:5<438::aid-lsm11>3.0.co;2-t
DO - 10.1002/(sici)1096-9101(1999)25:5<438::aid-lsm11>3.0.co;2-t
M3 - מאמר
C2 - 10602137
SN - 0196-8092
VL - 25
SP - 438
EP - 444
JO - Lasers in Surgery and Medicine
JF - Lasers in Surgery and Medicine
IS - 5
ER -