Effect of Leiurus quinquestriatus hebreus venom on calcium and deoxyglucose uptake in cultured cardiac cells

Galit Arie-Saadia, Shaul Sofer, Eli Zlotkin, Asher Shainberg

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

The effects of scorpion venom Leiurus quinquestriatus hebreus were studied on cardiac cells grown in culture. The venom (30 μg/ml) increased significantly (P < 0.05) Ca2+ uptake into intact cardiocytes and to sarcoplasmic reticulum of skinned cells. [3H]Deoxyglucose uptake was also increased significantly (P < 0.05) in venom treated cardiocytes. It was found that fractions I and III of the venom, separated by gel filtration and ion exchange chromatography, are responsible for the increased Ca2+ uptake by the sarcoplasmic reticulum, whereas fraction IIb, III and IV are responsible for the accelerated rate of uptake of 45Ca and [3H]deoxyglucose by intact cells. Ca channel blockers prevented these effects and similar results were obtained by propranolol. Thus, it is concluded that the venom exerts its effect through activation of β-adrenoceptors which causes the opening of L-type Ca channels.

Original languageEnglish
Pages (from-to)435-442
Number of pages8
JournalToxicon
Volume34
Issue number4
DOIs
StatePublished - 1 Jan 1996
Externally publishedYes

ASJC Scopus subject areas

  • Toxicology

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