Effect of surface parameters on the performance of IgG-arrayed hydrogel chips: A comprehensive study

Katarzyna Derwinska; Levi A. Gheber; Ursula Sauer; Leopold Schorn; Claudia Preininger

doi:10.1021/la700608c

Effect of surface parameters on the performance of IgG-arrayed hydrogel chips: A comprehensive study

Katarzyna Derwinska, Levi A. Gheber, Ursula Sauer, Leopold Schorn, Claudia Preininger

Department of Biotechnology Engineering

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

In this article, the assay performance of 3D polyurethane (PU) hydrogel surfaces, used either plain or modified with cross linkers and additives in a direct immunoassay for IgG, is correlated with chip surface parameters such as water content and expansion, mechanical stability, hydrophilicity, thickness, and surface topography. Commercial chip surfaces ARChip Epoxy, Nexterion slide H and HydroGel are used as references. A strong correlation between assay sensitivity and physical surface parameters was found only for various hydrogels of the same chemical composition, in which cases assay sensitivity increases with decreasing hydrogel concentration as well as decreasing roughness, water content, and expansion. However, as is the case with all hydrogels tested, more hydrophobic layers with low water content are more highly reproducible from one measurement to another.

Original language	English
Pages (from-to)	10551-10558
Number of pages	8
Journal	Langmuir
Volume	23
Issue number	21
DOIs	https://doi.org/10.1021/la700608c
State	Published - 9 Oct 2007

ASJC Scopus subject areas

General Materials Science
Condensed Matter Physics
Surfaces and Interfaces
Spectroscopy
Electrochemistry

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AU - Schorn, Leopold

AU - Preininger, Claudia

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AB - In this article, the assay performance of 3D polyurethane (PU) hydrogel surfaces, used either plain or modified with cross linkers and additives in a direct immunoassay for IgG, is correlated with chip surface parameters such as water content and expansion, mechanical stability, hydrophilicity, thickness, and surface topography. Commercial chip surfaces ARChip Epoxy, Nexterion slide H and HydroGel are used as references. A strong correlation between assay sensitivity and physical surface parameters was found only for various hydrogels of the same chemical composition, in which cases assay sensitivity increases with decreasing hydrogel concentration as well as decreasing roughness, water content, and expansion. However, as is the case with all hydrogels tested, more hydrophobic layers with low water content are more highly reproducible from one measurement to another.

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Effect of surface parameters on the performance of IgG-arrayed hydrogel chips: A comprehensive study

Abstract

ASJC Scopus subject areas

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