Effect of surface parameters on the performance of IgG-arrayed hydrogel chips: A comprehensive study

Katarzyna Derwinska, Levi A. Gheber, Ursula Sauer, Leopold Schorn, Claudia Preininger

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

In this article, the assay performance of 3D polyurethane (PU) hydrogel surfaces, used either plain or modified with cross linkers and additives in a direct immunoassay for IgG, is correlated with chip surface parameters such as water content and expansion, mechanical stability, hydrophilicity, thickness, and surface topography. Commercial chip surfaces ARChip Epoxy, Nexterion slide H and HydroGel are used as references. A strong correlation between assay sensitivity and physical surface parameters was found only for various hydrogels of the same chemical composition, in which cases assay sensitivity increases with decreasing hydrogel concentration as well as decreasing roughness, water content, and expansion. However, as is the case with all hydrogels tested, more hydrophobic layers with low water content are more highly reproducible from one measurement to another.

Original languageEnglish
Pages (from-to)10551-10558
Number of pages8
JournalLangmuir
Volume23
Issue number21
DOIs
StatePublished - 9 Oct 2007

Fingerprint

Dive into the research topics of 'Effect of surface parameters on the performance of IgG-arrayed hydrogel chips: A comprehensive study'. Together they form a unique fingerprint.

Cite this