Effect of wax degrading bacteria on life cycle of the pink hibiscus mealybug, Maconellicoccus hirsutus (Green) (Hemiptera: Pseudococcidae)

Rahul B. Salunkhe, Chandrashekhar D. Patil, Bipinchandra K. Salunke, Ninfa M. Rosas-García, Satish V. Patil

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

The pink mealybug Maconellicoccus hirsutus (Green) (Hemiptera: Pseudococcidae) is a polyphagous insect pest with protective wax covering. Bacterial isolates originating from M. hirsutus cadavers were screened for their wax degrading effects and the three most potent wax degrading isolates were identified to be Serratia marcescens, Pseudomonas aeruginosa and Bacillus subtilis by 16S rRNA gene sequencing. Bacteria degrading M. hirsutus wax reduced female longevity, offspring production as well as weight and wax content of emerging adults. Treatment with S. marcescens was the most effective in reducing wax content, fecundity and honeydew production. Analysis of honeydew produced by M. hirsutus after each microbial treatment revealed reduction in amino acids, proteins, and reducing sugars concentration compared to controls. Most negative effect on reducing sugars was observed in the B. subtilis treatment suggesting disequilibrium in mealybug metabolism. S. marcescens and P. aeruginosa treatments showed significantly lower content of wax as compared to control suggesting utilization of wax as carbon source by these microorganisms. This study suggests that the use of each of three bacterial isolates reduces wax content of mealybug and might be useful in devising mealybug control strategies.

Original languageEnglish
Pages (from-to)535-542
Number of pages8
JournalBioControl
Volume58
Issue number4
DOIs
StatePublished - 1 Aug 2013
Externally publishedYes

Keywords

  • Amino acids
  • Bacillus subtilis
  • Biocontrol
  • Honeydew
  • Pink hibiscus mealybug
  • Pseudomonas aeruginosa
  • Serratia marcescens

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Insect Science

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