Effects of light intensity and nitrogen starvation on growth, total fatty acids and arachidonic acid in the green microalga Parietochloris incisa

A. E. Solovchenko; I. Khozin-Goldberg; S. Didi-Cohen; Z. Cohen; M. N. Merzlyak

doi:10.1007/s10811-007-9233-0

Effects of light intensity and nitrogen starvation on growth, total fatty acids and arachidonic acid in the green microalga Parietochloris incisa

A. E. Solovchenko, I. Khozin-Goldberg, S. Didi-Cohen, Z. Cohen, M. N. Merzlyak

Research output: Contribution to journal › Article › peer-review

300 Scopus citations

Abstract

The effects of light and nitrogen deficiency on biomass, fatty acid content and composition were studied in Parietochloris incisa, the unicellular freshwater chlorophyte accumulating very high amounts of arachidonic-acid-rich triacylglycerols. P. incisa cultures grown on complete nutrient medium and under high light (400 μmol photons m^{- 2} s^-1) showed the highest rate of growth in comparison to medium (200 μmol photons m ^-2 s^-1) and low (35 μmol photons m^-2 s ^-1) light intensity. Cultures grown under high light (on complete BG-11 medium) attained higher volumetric contents of total fatty acids and arachidonic acid due to greater increase in biomass. Nitrogen starvation brought about a strong increase in the arachidonic acid proportion of total fatty acids. Thus, adjustments to cultivation conditions could serve as an efficient tool for manipulation of yield and relative content of arachidonic acid in P. incisa. The significance of the changes in lipid metabolism for adaptation of P. incisa to high-light stress and nitrogen deficiency is also discussed.

Original language	English
Pages (from-to)	245-251
Number of pages	7
Journal	Journal of Applied Phycology
Volume	20
Issue number	3
DOIs	https://doi.org/10.1007/s10811-007-9233-0
State	Published - 1 Jun 2008

Keywords

Alga cultivation
Lipids
Microalgal biotechnology
Photoadaptation

ASJC Scopus subject areas

Aquatic Science
Plant Science

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10.1007/s10811-007-9233-0

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title = "Effects of light intensity and nitrogen starvation on growth, total fatty acids and arachidonic acid in the green microalga Parietochloris incisa",

abstract = "The effects of light and nitrogen deficiency on biomass, fatty acid content and composition were studied in Parietochloris incisa, the unicellular freshwater chlorophyte accumulating very high amounts of arachidonic-acid-rich triacylglycerols. P. incisa cultures grown on complete nutrient medium and under high light (400 μmol photons m- 2 s-1) showed the highest rate of growth in comparison to medium (200 μmol photons m -2 s-1) and low (35 μmol photons m-2 s -1) light intensity. Cultures grown under high light (on complete BG-11 medium) attained higher volumetric contents of total fatty acids and arachidonic acid due to greater increase in biomass. Nitrogen starvation brought about a strong increase in the arachidonic acid proportion of total fatty acids. Thus, adjustments to cultivation conditions could serve as an efficient tool for manipulation of yield and relative content of arachidonic acid in P. incisa. The significance of the changes in lipid metabolism for adaptation of P. incisa to high-light stress and nitrogen deficiency is also discussed.",

keywords = "Alga cultivation, Lipids, Microalgal biotechnology, Photoadaptation",

author = "Solovchenko, {A. E.} and I. Khozin-Goldberg and S. Didi-Cohen and Z. Cohen and Merzlyak, {M. N.}",

note = "Funding Information: Acknowledgements This work was supported in part by fellowships from the BIDR to M.N.M. and A.E.S. M.N.M. and A.E.S. also acknowledge the financial support of the Russian Fund for Basic Research (Grant # 06-04-48883) and Russia President{\textquoteright}s Grant Council (Ministry of Science of the Russian Federation).",

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AU - Didi-Cohen, S.

AU - Cohen, Z.

AU - Merzlyak, M. N.

N1 - Funding Information: Acknowledgements This work was supported in part by fellowships from the BIDR to M.N.M. and A.E.S. M.N.M. and A.E.S. also acknowledge the financial support of the Russian Fund for Basic Research (Grant # 06-04-48883) and Russia President’s Grant Council (Ministry of Science of the Russian Federation).

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Y1 - 2008/6/1

N2 - The effects of light and nitrogen deficiency on biomass, fatty acid content and composition were studied in Parietochloris incisa, the unicellular freshwater chlorophyte accumulating very high amounts of arachidonic-acid-rich triacylglycerols. P. incisa cultures grown on complete nutrient medium and under high light (400 μmol photons m- 2 s-1) showed the highest rate of growth in comparison to medium (200 μmol photons m -2 s-1) and low (35 μmol photons m-2 s -1) light intensity. Cultures grown under high light (on complete BG-11 medium) attained higher volumetric contents of total fatty acids and arachidonic acid due to greater increase in biomass. Nitrogen starvation brought about a strong increase in the arachidonic acid proportion of total fatty acids. Thus, adjustments to cultivation conditions could serve as an efficient tool for manipulation of yield and relative content of arachidonic acid in P. incisa. The significance of the changes in lipid metabolism for adaptation of P. incisa to high-light stress and nitrogen deficiency is also discussed.

AB - The effects of light and nitrogen deficiency on biomass, fatty acid content and composition were studied in Parietochloris incisa, the unicellular freshwater chlorophyte accumulating very high amounts of arachidonic-acid-rich triacylglycerols. P. incisa cultures grown on complete nutrient medium and under high light (400 μmol photons m- 2 s-1) showed the highest rate of growth in comparison to medium (200 μmol photons m -2 s-1) and low (35 μmol photons m-2 s -1) light intensity. Cultures grown under high light (on complete BG-11 medium) attained higher volumetric contents of total fatty acids and arachidonic acid due to greater increase in biomass. Nitrogen starvation brought about a strong increase in the arachidonic acid proportion of total fatty acids. Thus, adjustments to cultivation conditions could serve as an efficient tool for manipulation of yield and relative content of arachidonic acid in P. incisa. The significance of the changes in lipid metabolism for adaptation of P. incisa to high-light stress and nitrogen deficiency is also discussed.

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Effects of light intensity and nitrogen starvation on growth, total fatty acids and arachidonic acid in the green microalga Parietochloris incisa

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