Effects of temperature inactivation of penicillin-binding protein 2 on envelope growth in Escherichia coli

D. Buchnik; C. L. Woldringh; A. Zaritsky

doi:10.1016/0769-2609(87)90039-1

Effects of temperature inactivation of penicillin-binding protein 2 on envelope growth in Escherichia coli

D. Buchnik, C. L. Woldringh, A. Zaritsky

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

The transition from rod-shaped to spheroidal cells was studied in a temperature-sensitive strain (SP45) of Escherichia coli K12, carrying a mutation (pbpA) in the gene coding for penicillin-binding protein 2 (PBP-2). This transition imposed by the restrictive temperature was associated with reduction of peptidoglycan/surface area and of cellular osmotic stability. Addition of nalidixic acid (20 μg/ml) at the temperature shift from 30 to 42°C resulted in lysis of some cells and appearance of spheroidal bulges along the cylinders in other cells, consistent with the hypothesis of envelope weakening due to inactivation of PBP-2.

Original language	English
Pages (from-to)	537-547
Number of pages	11
Journal	Annales de l'Institut Pasteur Microbiology
Volume	138
Issue number	5
DOIs	https://doi.org/10.1016/0769-2609(87)90039-1
State	Published - 1 Jan 1987

Keywords

Cell wall
Escherichia coli
Inactivation
PBP-2
Penicillin
Protein
Spheroidal form

ASJC Scopus subject areas

Medicine (all)

Access to Document

10.1016/0769-2609(87)90039-1

Cite this

@article{a4299cfde6964cb29eb0c281b1aa23e4,

title = "Effects of temperature inactivation of penicillin-binding protein 2 on envelope growth in Escherichia coli",

abstract = "The transition from rod-shaped to spheroidal cells was studied in a temperature-sensitive strain (SP45) of Escherichia coli K12, carrying a mutation (pbpA) in the gene coding for penicillin-binding protein 2 (PBP-2). This transition imposed by the restrictive temperature was associated with reduction of peptidoglycan/surface area and of cellular osmotic stability. Addition of nalidixic acid (20 μg/ml) at the temperature shift from 30 to 42°C resulted in lysis of some cells and appearance of spheroidal bulges along the cylinders in other cells, consistent with the hypothesis of envelope weakening due to inactivation of PBP-2.",

keywords = "Cell wall, Escherichia coli, Inactivation, PBP-2, Penicillin, Protein, Spheroidal form",

author = "D. Buchnik and Woldringh, {C. L.} and A. Zaritsky",

year = "1987",

month = jan,

day = "1",

doi = "10.1016/0769-2609(87)90039-1",

language = "English",

volume = "138",

pages = "537--547",

journal = "Annales de l'Institut Pasteur Microbiology",

issn = "0769-2609",

publisher = "Elsevier BV",

number = "5",

}

TY - JOUR

T1 - Effects of temperature inactivation of penicillin-binding protein 2 on envelope growth in Escherichia coli

AU - Buchnik, D.

AU - Woldringh, C. L.

AU - Zaritsky, A.

PY - 1987/1/1

Y1 - 1987/1/1

N2 - The transition from rod-shaped to spheroidal cells was studied in a temperature-sensitive strain (SP45) of Escherichia coli K12, carrying a mutation (pbpA) in the gene coding for penicillin-binding protein 2 (PBP-2). This transition imposed by the restrictive temperature was associated with reduction of peptidoglycan/surface area and of cellular osmotic stability. Addition of nalidixic acid (20 μg/ml) at the temperature shift from 30 to 42°C resulted in lysis of some cells and appearance of spheroidal bulges along the cylinders in other cells, consistent with the hypothesis of envelope weakening due to inactivation of PBP-2.

AB - The transition from rod-shaped to spheroidal cells was studied in a temperature-sensitive strain (SP45) of Escherichia coli K12, carrying a mutation (pbpA) in the gene coding for penicillin-binding protein 2 (PBP-2). This transition imposed by the restrictive temperature was associated with reduction of peptidoglycan/surface area and of cellular osmotic stability. Addition of nalidixic acid (20 μg/ml) at the temperature shift from 30 to 42°C resulted in lysis of some cells and appearance of spheroidal bulges along the cylinders in other cells, consistent with the hypothesis of envelope weakening due to inactivation of PBP-2.

KW - Cell wall

KW - Escherichia coli

KW - Inactivation

KW - PBP-2

KW - Penicillin

KW - Protein

KW - Spheroidal form

UR - http://www.scopus.com/inward/record.url?scp=0023626671&partnerID=8YFLogxK

U2 - 10.1016/0769-2609(87)90039-1

DO - 10.1016/0769-2609(87)90039-1

M3 - Article

AN - SCOPUS:0023626671

SN - 0769-2609

VL - 138

SP - 537

EP - 547

JO - Annales de l'Institut Pasteur Microbiology

JF - Annales de l'Institut Pasteur Microbiology

IS - 5

ER -

Effects of temperature inactivation of penicillin-binding protein 2 on envelope growth in Escherichia coli

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this