Effects of temperature inactivation of penicillin-binding protein 2 on envelope growth in Escherichia coli

D. Buchnik; C. L. Woldringh; A. Zaritsky

doi:10.1016/0769-2609(87)90039-1

Effects of temperature inactivation of penicillin-binding protein 2 on envelope growth in Escherichia coli

D. Buchnik, C. L. Woldringh, A. Zaritsky

Department of Life Sciences

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2 Scopus citations

Abstract

The transition from rod-shaped to spheroidal cells was studied in a temperature-sensitive strain (SP45) of Escherichia coli K12, carrying a mutation (pbpA) in the gene coding for penicillin-binding protein 2 (PBP-2). This transition imposed by the restrictive temperature was associated with reduction of peptidoglycan/surface area and of cellular osmotic stability. Addition of nalidixic acid (20 μg/ml) at the temperature shift from 30 to 42°C resulted in lysis of some cells and appearance of spheroidal bulges along the cylinders in other cells, consistent with the hypothesis of envelope weakening due to inactivation of PBP-2.

Original language	English
Pages (from-to)	537-547
Number of pages	11
Journal	Annales de l'Institut Pasteur Microbiology
Volume	138
Issue number	5
DOIs	https://doi.org/10.1016/0769-2609(87)90039-1
State	Published - 1 Jan 1987

Keywords

Cell wall
Escherichia coli
Inactivation
PBP-2
Penicillin
Protein
Spheroidal form

ASJC Scopus subject areas

General Medicine

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10.1016/0769-2609(87)90039-1

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title = "Effects of temperature inactivation of penicillin-binding protein 2 on envelope growth in Escherichia coli",

abstract = "The transition from rod-shaped to spheroidal cells was studied in a temperature-sensitive strain (SP45) of Escherichia coli K12, carrying a mutation (pbpA) in the gene coding for penicillin-binding protein 2 (PBP-2). This transition imposed by the restrictive temperature was associated with reduction of peptidoglycan/surface area and of cellular osmotic stability. Addition of nalidixic acid (20 μg/ml) at the temperature shift from 30 to 42°C resulted in lysis of some cells and appearance of spheroidal bulges along the cylinders in other cells, consistent with the hypothesis of envelope weakening due to inactivation of PBP-2.",

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T1 - Effects of temperature inactivation of penicillin-binding protein 2 on envelope growth in Escherichia coli

AU - Buchnik, D.

AU - Woldringh, C. L.

AU - Zaritsky, A.

PY - 1987/1/1

Y1 - 1987/1/1

N2 - The transition from rod-shaped to spheroidal cells was studied in a temperature-sensitive strain (SP45) of Escherichia coli K12, carrying a mutation (pbpA) in the gene coding for penicillin-binding protein 2 (PBP-2). This transition imposed by the restrictive temperature was associated with reduction of peptidoglycan/surface area and of cellular osmotic stability. Addition of nalidixic acid (20 μg/ml) at the temperature shift from 30 to 42°C resulted in lysis of some cells and appearance of spheroidal bulges along the cylinders in other cells, consistent with the hypothesis of envelope weakening due to inactivation of PBP-2.

AB - The transition from rod-shaped to spheroidal cells was studied in a temperature-sensitive strain (SP45) of Escherichia coli K12, carrying a mutation (pbpA) in the gene coding for penicillin-binding protein 2 (PBP-2). This transition imposed by the restrictive temperature was associated with reduction of peptidoglycan/surface area and of cellular osmotic stability. Addition of nalidixic acid (20 μg/ml) at the temperature shift from 30 to 42°C resulted in lysis of some cells and appearance of spheroidal bulges along the cylinders in other cells, consistent with the hypothesis of envelope weakening due to inactivation of PBP-2.

KW - Cell wall

KW - Escherichia coli

KW - Inactivation

KW - PBP-2

KW - Penicillin

KW - Protein

KW - Spheroidal form

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Effects of temperature inactivation of penicillin-binding protein 2 on envelope growth in Escherichia coli

Abstract

Keywords

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