TY - JOUR
T1 - Effects of Wnt1 signaling on proliferation in the developing mid-/hindbrain region
AU - Panhuysen, Markus
AU - Vogt Weisenhorn, Daniela M.
AU - Blanquet, Veronique
AU - Brodski, Claude
AU - Heinzmann, Ulrich
AU - Beisker, Wolfgang
AU - Wurst, Wolfgang
N1 - Funding Information:
We thank Drs. Laure Bally-Cuif, Jordi Guimera, Gail Martin, Nilima Prakash, and Martin Raff for critical scientific input; Rudolf Grosschedl, Peter Gruss, Alexandra L. Joyner, Antonello Mallamaci, Gail Martin, Salvador Martinez, Charles J. Sherr, Antonio Simeone, and Marion Wassef for plasmids and probes; Luise Jennen for electron microscopic assistance; Annerose Kurz-Drexler, Susanne Bourier, Stefanie Pirrung, Sava Michailidou for excellent technical assistance; and Resi Wandrowetz, Rosi Pfeiffer, Monika Nadler, and Mario Christan for animal husbandry. This work was supported in part by the BMBF (WW), the EU program Eumorphia (WW), and the Deutsche Forschungsgemeinschaft (WW).
PY - 2004/5/1
Y1 - 2004/5/1
N2 - The secreted glycoprotein WNT1 is expressed in the caudal midbrain and is essential for proper development of the entire mid-/hindbrain region. To get better insights into Wnt1 function in the mid-/hindbrain region, we ectopically expressed Wnt1 under the control of the endogenous En1 promoter, thereby extending Wnt1 expression rostrally into the anterior midbrain and caudally into rhombomere 1. In these transgenic mice, the position of the mid-/hindbrain organizer is not altered and pattern formation is not changed. During midgestation, ectopic Wnt1 induced strong overproliferation of precursor cells only in the caudal midbrain in a gene dosage-dependent manner. Enhanced proliferation is at least in part mediated by shortening of the cell cycle length. In adults, Wnt1 exhibited a cell size promoting effect specifically on neurons. We suggest that Wnt1 acts as a regulator of proliferation of specific precursor populations in the developing mid-/hindbrain region and is only secondarily involved in maintenance of the mid-/hindbrain organizer.
AB - The secreted glycoprotein WNT1 is expressed in the caudal midbrain and is essential for proper development of the entire mid-/hindbrain region. To get better insights into Wnt1 function in the mid-/hindbrain region, we ectopically expressed Wnt1 under the control of the endogenous En1 promoter, thereby extending Wnt1 expression rostrally into the anterior midbrain and caudally into rhombomere 1. In these transgenic mice, the position of the mid-/hindbrain organizer is not altered and pattern formation is not changed. During midgestation, ectopic Wnt1 induced strong overproliferation of precursor cells only in the caudal midbrain in a gene dosage-dependent manner. Enhanced proliferation is at least in part mediated by shortening of the cell cycle length. In adults, Wnt1 exhibited a cell size promoting effect specifically on neurons. We suggest that Wnt1 acts as a regulator of proliferation of specific precursor populations in the developing mid-/hindbrain region and is only secondarily involved in maintenance of the mid-/hindbrain organizer.
UR - http://www.scopus.com/inward/record.url?scp=2342514254&partnerID=8YFLogxK
U2 - 10.1016/j.mcn.2004.01.011
DO - 10.1016/j.mcn.2004.01.011
M3 - Article
AN - SCOPUS:2342514254
SN - 1044-7431
VL - 26
SP - 101
EP - 111
JO - Molecular and Cellular Neuroscience
JF - Molecular and Cellular Neuroscience
IS - 1
ER -